Ion AmpliSeq™ Sample ID Panel

Ion Torrent™

Ion AmpliSeq™ Sample ID Panel

The Ion AmpliSeq Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 speciallyRead more

Catalog Number	Quantity
4479790	96 reactions

Catalog number 4479790

Price (CNY)

Quantity:

96 reactions

The Ion AmpliSeq Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 specially designed primer pairs that can be added to the multiplex PCR reaction to generate a unique ID during post-sequencing analysis of research samples.

Designed to advance clinical research, the Ion AmpliSeq Sample ID Panel helps reveal sample misidentification for increased confidence during data analysis and reporting. The Ion AmpliSeq Sample ID Panel can be used in conjunction with any Ion AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.ampliseq.cn).

Verify various sample combinations:

Tumor/normal paired samples
Samples from the same individual in longitudinal studies
Multi-tissue or multi-tumor samples from the same individual

Sample identification through SNP genotyping

Panel includes nine primer pairs targeting nine (female samples) or ten (male samples) validated SNPs
SNPs are unlinked and exhibit exceptional robustness and consistently high minor allele frequency across a diverse group of human populations
One primer pair targets the amelogenin gene and amplifies one target on X chromosome and a distinct target on Y chromosome, allowing simple and quick sample gender determination
First letter of Sample ID code provides instant gender determination for sample
Optimal discrimination power (∼1:5,000*) with minimal sequencing needed

Simple workflow—just one pipetting step

Spike 1 μL of the 20X Sample ID primer pool directly into any Ion AmpliSeq primer pool for co-amplification and downstream sequencing and data analysis
Enabled for use with any Ion Xpress barcode
Compatible with the Ion Reporter Software, a cloud-hosted software tool for automated variant analysis

Includes:

20X Ion AmpliSeq Sample ID Primer Pool

Required:

Torrent Suite Software (v3.0 or greater) that includes the Ion AmpliSeq Sample ID Panel plug-in
Ion AmpliSeq Library Kit 2.0 User Guide
Ion AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.ampliseq.cn)

¹Pakstis AJ et al. SNPs for a Universal Individual Identification Panel. Hum Genet. 2010 Mar; 127(3):315-24.

*4641 discrimination power calculations assumes no missing genotyping data

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Sequencing

For Use With (Equipment)Ion PGM™ System, Ion Proton™ System, Ion GeneStudio™ S5 System

LibrariesTargeted Sequencing Library

Multiplex Capability9 amplicons

No. of Reactions96

PrimerSample ID Primers

Product LineIon AmpliSeq

Product TypeSample ID Panel

Quantity96 reactions

Sample TypeDNA

Shipping ConditionApproved for shipment at Room Temperature or on Dry Ice

SpeciesHuman

Starting Material Amount10 ng gDNA

TechniqueAmplicon Sequencing

Workflow StepSample QC

Sequencing TypeGenome and DNA Sequencing

Unit SizeEach

Contents & Storage

1 tube 20X Ion AmpliSeq™ Sample ID Primer Pool
Store at -30 °C to -10 °C

Frequently asked questions (FAQs)

What is the difference between a DNA fragment library and a DNA amplicon library?

A DNA fragment library is constructed from whole genomic DNA and is commonly used for whole genome resequencing or de novo sequencing. Briefly, the whole genomic DNA is fragmented or sheared, ligated with Ion-specific adapter sequences, and then size-selected for the library fragments of the desired length.

Amplicon libraries are constructed from PCR-amplified DNA fragments and are used for targeted sequencing (e.g., investigating variants at known genomic locations). There are two types of amplicon libraries, short and long.

A short amplicon library contains DNA fragments (targets) with lengths that are compatible with the Ion template preparation kits without any further shearing or fragmentation during library preparation. Additionally, no size-selection step is required, as the amplicons are already within the desired size range.

A long amplicon library contains DNA fragments (targets) with lengths that are longer than those compatible with the Ion template kits and requires further shearing or fragmentation during library preparation. The library preparation protocol for long amplicons is similar to fragment libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the Ion AmpliSeq Sample ID Panel? How is it used?

The Ion AmpliSeq Sample ID Panel (Cat. No. 4479790) is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 specially designed primer pairs that can be added to the target amplification reaction to generate a unique ID during post-sequencing analysis of research samples using the on AmpliSeq Sample ID Panel plug-in. This panel is useful for tracking tumor/normal paired samples, samples from the same individual in longitudinal studies, and multi-tissue or multi-tumor samples from the same individual.

The Ion AmpliSeq Sample ID Panel can be used in conjunction with all Ion AmpliSeq Ready-to-Use, Custom, and Community panels.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

My Ion Torrent Ion AmpliSeq custom design has low predicted coverage; what can I do to improve it?

If you've reviewed your design and are not satisfied with the results, please click on the “Not happy with this design? Let us help” link to have an Ion AmpliSeq team member contact you about additional options for your design.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Bioanalyzer instrument trace of my fragment library shows a large peak at 70 bp or 90 bp. What can cause this? Is it okay to proceed to template preparation?

The ~70 bp or ~90 bp peak is likely standard or barcoded adapter dimers, respectively. Adapter dimers may form during the adapter ligation step and are usually removed during the size selection process. The adapter dimers will amplify on the Ion Torrent Ion Sphere particles during template preparation and decrease the overall throughput of usable sequencing reads; thus, we highly recommend removing the adapter dimers by performing an additional clean-up step prior to template preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I would like to perform Next-Generation sequencing but my final library yield is poor. What are some tips for improving my yield?

In addition to input RNA quality and accurate quantification, the clean-up and size selection steps are critical to generating a successful RNA-Seq library.

- Be sure to mix the nucleic acid binding beads well before dispensing, and follow the workflow and incubation times as closely as possible.
- Use fresh ethanol and pre-wet pipette tips prior to transferring ethanol, as the volume is critical for size selection.
- Remove residual ethanol before elution using a small-volume pipette. Do not over-dry or under-dry the beads.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

View all Ion AmpliSeq™ Sample ID Panel FAQs