Ion Library Equalizer™ Kit

The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and libraryRead more

Catalog Number	Quantity
4482298	96 reactions

Catalog number 4482298

Price (CNY)

Quantity:

96 reactions

The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.

The kit workflow consists of three simple steps:

1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer

Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability

Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency

Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization

Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.

Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.cn)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit

Optional:
Ion Xpress™ Barcode Adapters Kits

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Amplicon Sequencing

For Use With (Equipment)Ion PGM™ System, Ion Proton™ System, Ion Personal Genome Machine (PGM™) System

Format96-well Plate

LibrariesFragment Library, Targeted Sequencing Library, Normalized Library

Multiplex CapabilityNo Limit on Multiplexing

Product TypeLibrary Equalizer Kit

Quantity96 reactions

Sample TypeAny Sample Type

Sample VolumeFragment or Targeted AmpliSeq Libraries

Sequencing TypeEpigenetic Sequencing, Genome & DNA Sequencing

SpeciesAll Species

ThroughputUp to 10 Samples

Workflow StepLibrary Barcoding, Library Generation, Template Preparation

Product LineEqualizer

Starting MaterialDNA

Unit SizeEach

Contents & Storage

• Equalizer Primers - store at 2–8°C
• Equalizer Capture - store at 2–8°C
• Equalizer Wash Buffer - store at room temperature (can be stored at 2–8°C)
• Equalizer Elution Buffer - store at 2–8°C
• Equalizer Beads - store at 2–8°C

Note kits are shipped at ambient temperature store each component at specified temperatures.

Frequently asked questions (FAQs)

Can the Ion Library Equalizer Kit (Cat. No. 4482298) be used to normalize 400-base read libraries?

Currently, the Ion Library Equalizer Kit is validated and only recommended for use with up to 300-base read libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is the Ion Library Equalizer Kit compatible with Ion Ampliseq libraries?

The Ion Library Equalizer Kit (Cat. No. 4482298) is recommended for use with Ion AmpliSeq libraries and provides an alternative to library quantification methods by using bead-based technology to normalize the final library concentration to ~100 pM.

The Ion Library Equalizer Kit is fast and cost-effective compared to traditional quantification methods; however, it may be not be the right choice for all users. Briefly, the library is amplified with the Ion Equalizer Primers, captured onto Equalizer Beads, and the normalized library is eluted from the beads using a specially formulated Equalizer Elution Buffer. The final library is normalized to ~100 pM, but there is no quality control information (e.g., measured concentration or size distribution) that can be obtained, which is possible if using the recommended library quantification kits: Ion Library Quantitation Kit (qPCR), Qubit dsDNA HS Assay Kit (Qubit 2.0 Fluorometer), or Agilent High Sensitivity DNA Kit (Agilent Bioanalyzer instrument).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I am planning to use the Oncomine BRCA Research Assay, Manual Library Preparation (Cat. No. A32840). Which other products would need I to order?

Here are the products you would need to order:

- Ion Xpress Barcode Adapters Kits
- Agencourt AMPure XP (Beckman Coulter Cat. No. A63880 or A63881)
- Ion Library Equalizer Kit (Cat. No. 4482298)