POROS™ CaptureSelect™ GCSF Affinity Column, 4.6 x 100 mm
POROS™ CaptureSelect™ GCSF Affinity Column, 4.6 x 100 mm
Thermo Scientific™

POROS™ CaptureSelect™ GCSF Affinity Column, 4.6 x 100 mm

Thermo Scientific™ POROS™ CaptureSelect™ GCSF HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.
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Catalog NumberQuantity
44851681 column
Catalog number 4485168
Price (CNY)
41,344.00
Each
Add to cart
Quantity:
1 column
Price (CNY)
41,344.00
Each
Add to cart

Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS CaptureSelect GCSF HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands. The combination allows three-minute quantitation of granulocyte colony-stimulating factor (GCSF).

  • Broad utility—POROS A, G, and CaptureSelect affinity ligands address most biological drug classes
  • Rapid—POROS resins offer fast quantitation or purification of immunoglobulins, fusion proteins
  • Hands-off operation—HPLC method can be automated for streamlined operation
  • Robust—allows for high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity

The combination of CaptureSelect GCSF affinity ligand and POROS 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid titer determination and small-scale purification of GCSF.

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.

Automation

POROS affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeAffinity
Elution Volume Max29 column volumes
Elution Volume Min14 column volumes
High-throughput CompatibilityAutomated Protocols
MatrixPOROS
Max. Pressure2600 psi (180 bar)
Quantity1 column
Stationary PhaseCaptureSelect™ GCSF
Target MoleculeProtein
Volume (Metric)1.7 mL
Particle Size20 μm
Product LineCaptureSelect, POROS
TypePrepacked Column
Unit SizeEach
Contents & Storage
Column includes EZ grip fittings and column test certificate.
Store at 2°C to 8°C. DO NOT FREEZE.

Frequently asked questions (FAQs)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all POROS™ CaptureSelect™ GCSF Affinity Column, 4.6 x 100 mm FAQs