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Thermo Scientific™
SulfoLink™ Immobilization Kit for Proteins, 2 mL
Thermo Scientific SulfoLink Immobilization Kit for Proteins, 2 mL is a kit containing all the reagents for preparing the proteinRead more
| Catalog Number | Quantity |
|---|---|
| 44995 | 5 Column Kit |
Catalog number 44995
Price (CNY)
9,398.00
Each
Quantity:
5 Column Kit
Price (CNY)
9,398.00
Each
Thermo Scientific SulfoLink Immobilization Kit for Proteins, 2 mL is a kit containing all the reagents for preparing the protein sample for efficient immobilization and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.
Features of SulfoLink Immobilization Kit for Proteins, 2 mL:
• Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Fast—spin columns increase protocol speed; prepare and couple protein samples in 3.5 hours
• Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin
Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
When incubated with a solution of protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Related Products
SulfoLink™ Coupling Resin
SulfoLink™ Immobilization Kit for Peptides, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL
Features of SulfoLink Immobilization Kit for Proteins, 2 mL:
• Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Fast—spin columns increase protocol speed; prepare and couple protein samples in 3.5 hours
• Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin
Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
When incubated with a solution of protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Related Products
SulfoLink™ Coupling Resin
SulfoLink™ Immobilization Kit for Peptides, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeAffinity, Agarose Resin
FormatSpin Column
Quantity5 Column Kit
Stationary PhaseHaloacetyl
Sufficient For2 to 20 mg of Antibody per Column
Capacity (Metric)2 mL
Product LineSulfoLink™
TypeImmobilization Kit
Unit SizeEach
Contents & Storage
Sufficient For: Immobilizing 1 to 10 mg of antibody per column
• SulfoLink Columns, 2 mL, 5 columns
• SulfoLink Preparation Buffer, 7.5 mL
• SulfoLink Coupling Buffer, 500 mL
• Wash Solution, 120 mL
• PBS Pack (makes 500 mL), 1 pack
• 2-Mercaptoethylamine·HCl, 5 x 6 mg
• L-Cysteine·HCl, 100 mg
• Zeba Spin Desalting Columns, 5 mL, 5 columns
• Column Accessories
Store at 4°C protected from light.
• SulfoLink Columns, 2 mL, 5 columns
• SulfoLink Preparation Buffer, 7.5 mL
• SulfoLink Coupling Buffer, 500 mL
• Wash Solution, 120 mL
• PBS Pack (makes 500 mL), 1 pack
• 2-Mercaptoethylamine·HCl, 5 x 6 mg
• L-Cysteine·HCl, 100 mg
• Zeba Spin Desalting Columns, 5 mL, 5 columns
• Column Accessories
Store at 4°C protected from light.