TaqMan™ TAMRA Probe
TaqMan™ TAMRA Probe
Citations & References (1)
Applied Biosystems™

TaqMan™ TAMRA Probe

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Applied Biosystems TaqMan TAMRA probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan TAMRA Probes featureRead more
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Catalog NumberQuantityProduct Type
45000350,000 pmolTAMRA Probe
45002420,000 pmolTAMRA Probe
4500256,000 pmolTAMRA Probe
Catalog number 450003
Price (CNY)
17,617.00
Each
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Quantity:
50,000 pmol
Product Type:
TAMRA Probe
Applied Biosystems TaqMan TAMRA probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan TAMRA Probes feature a 5’ fluorescent reporter dye (FAM, VIC, or TET) and 3’ fluorescent quencher (TAMRA dye). TaqMan TAMRA probes, which were some of the first TaqMan probes to be developed, can be used for a variety of applications.

TAMRA is a fluorescent dye and can be multiplexed with up to three dyes. (If multiplexing higher than triplex, TaqMan QSY probes are recommended.) TAMRA probes are typically longer (30 to >40 bases). For enhanced specificity, shorter TaqMan MGB nonfluorescent quencher (NFQ) probes (generally 15 to 20 bases) are also available.

All TaqMan TAMRA probes are HPLC-purified and verified by mass spectrometry to help ensure lot-to-lot reproducibility between batches. Thermo Fisher Scientific has been a leader in qPCR probe manufacturing for over 20 years. TaqMan Probes are manufactured in a facility that is certified to ISO 13485.

Guaranteed yield
The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.

Approximate ship time
4–6 days in North America, 5-10 days in Europe

We also offer Applied Biosystems custom primers and other types of TaqMan probes. Learn more ›

Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs. Learn more ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
3'Primer ModificationTAMRA
5'Primer ModificationFAM, TET, VIC
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
FormatTube
Green FeaturesSustainable packaging
Internal Probe ModificationNone
Product LineTaqMan
Product TypeTAMRA Probe
Purification MethodHPLC
Purity or Quality GradeHPLC
Quantity50,000 pmol
Shipping ConditionRoom Temperature
For Use With (Application)Real Time PCR (qPCR)
FormLiquid or Dried Down
Unit SizeEach
Contents & Storage
1 tube containing a dual-labeled probe in solution or dried down.

Store at -15°C to -25°C

Frequently asked questions (FAQs)

I would like to calibrate my Real-Time PCR instrument with a custom dye, which buffer should I use?

Please ask your dye manufacturer for recommendations. If there is no specific recommendation available, try TE Buffer (pH 8.0) to start.

I need to see all the primer and probe sequences from an assay design right away. How can I do this?

You can directly see all the primer and probe sequences from a test design right away using the Primer Express Software. Open the software and choose File => New. Choose either TaqMan MGB Quantification or TaqMan Quantification for Gene Expression Assay design. Copy/paste your gene sequence of interest into the new window and click on the green triangle to get primer/probe designs.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I determine whether the Tm of my custom probe is appropriate?

In general, the target Tm of a TaqMan probe is 70 degrees C. Using the Primer Express Software, you can check the Tm of a MGB or TAMRA probe probe.

Within the software, go to Primer Probe Test Tool and then copy/paste in your sequence of interest to see the Tm. If you need to check for a MGB based probe, make sure to choose Document Type: TaqMan MGB Quantification. If you need to check for a QSY or TAMRA based probe, make sure to choose Document Type: TaqMan Quantification. Real-Time PCR and Digital PCR Applications

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

I have purchased custom oligos (primers and probes) and I want to make a 20x working stock for a TaqMan assay, similar to the pre-designed assays. How do I dilute and mix my oligos to make a 20x working stock?

Most pre-designed TaqMan assays are supplied as a 20x assay concentrate containing 18 uM of each primer and 5 uM of probe which provides a 900 nM of each primer and 250 nM of the probe (for gene expression assays) and 200 nM of each probe (for SNP genotyping assays) at the final 1x concentration.

In order to make a 20x working stock from custom oligos, the oligos need to be diluted as follows: To make a 20x Assay stock for Gene Expression assays, make 100 uM stocks of both forward and reverse primers, and TaqMan probe. Add 18 ul of each primer and 5 ul of the TaqMan probe, which gives 41 ul volume. Add 59 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan Gene Expression Assay. To make a 20x Assay stock for a Genotyping Assay, make 100 uM stocks of both forward and reverse primers, and both TaqMan probes. Add 18 ul of each primer and 4 ul of each TaqMan probe, which gives 44 ul volume. Add 56 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan SNP Genotyping Assays.

I am looking to order a molecular probe for validation, using specific sequences. How do I order a custom probe?

We do not offer any custom probes for diagnostic purposes.

We do offer custom TaqMan probes for qPCR experiments, which are for Research Use Only.
TaqMan MGB Probe
TaqMan QSY Probe
TaqMan TAMRA Probe
These above probes can be designed in our online tool, Custom TaqMan Probes.

If you would like to order a pre-mix of primers and probe, please check our custom TaqMan Assays.
You can find more information one the Custom TaqMan Gene Expression Assay page on thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

View all TaqMan™ TAMRA Probe, 50,000 pmol FAQs

Citations & References (1)

Citations & References
Abstract
Presenilin-dependent gamma-secretase-like intramembrane cleavage of ErbB4.
Authors: Lee Hahn-Jun; Jung Kwang-Mook; Huang Yang Z; Bennett Lori B; Lee Joanne S; Mei Lin; Kim Tae-Wan;
Journal:J Biol Chem
PubMed ID:11741961
An unusual protease gamma-secretase requires functional presenilins and cleaves substrates (e.g. amyloid beta-protein precursor and Notch) with very loose amino acid sequence specificity within the transmembrane region. Here we report that ErbB4, a tyrosine kinase receptor for neuregulins, is a substrate for presenilin-dependent gamma-secretase. Our studies show that constitutive ectodomain ... More