MEM, no glutamine, no phenol red
Product Image
Gibco™

MEM, no glutamine, no phenol red

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be usedRead more
Have Questions?
Catalog NumberQuantity
51200087
also known as 51200-087
10 x 500 mL
Catalog number 51200087
also known as 51200-087
Price (CNY)
-
Quantity:
10 x 500 mL
Customize this product

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM is modified as follows:
Without
• L-glutamine
• Phenol Red
• HEPES


The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality280 - 330 mOsm/kg
Product LineGibco™
Product TypeMinimum Essential Medium (MEM)
Quantity10 x 500 mL
Shelf Life12 Months
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesSodium Bicarbonate
Without AdditivesNo Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
pH7 to 7.4
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Frequently asked questions (FAQs)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.