Fluoroskan Ascent™ Microplate Fluorometer
Fluoroskan Ascent™ Microplate Fluorometer
Actual product may vary
Thermo Scientific™

Fluoroskan Ascent™ Microplate Fluorometer

Perform a variety of research applications with the compact, robust Thermo Scientific™ Fluoroskan Ascent™ Microplate Fluorometer.
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Catalog NumberNo. of Dispensers
5210470Up to 3 Optional
Catalog number 5210470
Price (CNY)
-
No. of Dispensers:
Up to 3 Optional

Perform a variety of research applications with the compact and robust Thermo Scientific™ Fluoroskan Ascent™ Microplate Fluorometer. Featuring excellent optical performance, this microplate fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, immuno and cell based assays.

Onboard dispensers for exact follow-up of kinetic reactions

  • Comes equipped with up to three reagent dispensers for fast kinetic assays, such as Ca2+ flux
  • Supports simultaneous dispensing and reading, enabling monitoring of fast kinetic measurements from the very start of the reaction
  • Allows conservation of expensive reagents with very low dead volume and the backflush capability

High sensitivity for both top and bottom reading

  • Fiberless direct illumination optics for both top and bottom reading
  • Ensures a high sensitivity, a wide dynamic range and accurate and precise results for 1- to 384-well plates

Specially designed for automation

  • Reads up to 384-well plates
  • Easily integrates with automated systems
  • Enables the most rapid throughput

Includes:

PC Software and filter pairs: Excitation: 355nm/Emission: 460nm, Excitation: 485nm/ Emission: 538nm

Recommended for:

Ca2+ flux assays; Cell proliferation; Cytotoxicity; Multi-drug resistance; Cell adhesion; DNA quantitation; Reporter gene assays; Hybridization assays; Ion Channel; Quantitation of PCR products; FRET assays; Molecular beacon assays; Immunoassays; Enzyme activity; Neonatalogy; Bacterial quantitation; Phagocytosis; Oligonucleotide assays; ADMEtox

Specifications
Depth (English)16.5 in.
Depth (Metric)42 cm
Dynamic Range>6 decades
Emission FiltersUp to eight filters in the emission filter wheel. 460 and 538nm filters included as standard.
Emission Wavelength Range360 to 800 nm
Excitation FiltersUp to eight filters in the excitation filter wheel. 355 and 485nm filters included as standard.
Excitation Wavelength Range320 to 700 nm
Height (English)13.4 in.
Height (Metric)34 cm
IncludesPC Software and filter pairs: Excitation: 355nm/Emission: 460nm, Excitation: 485nm/ Emission: 538nm
Measurement Speed96 wells: 15 sec.
No. of DispensersUp to 3 Optional
Sensitivity2fmol fluorescein/well in a black 96-well plate
Warranty1 Year
Weight (English)46 lb.
Weight (Metric)21 kg
Width (English)16.5 in.
Width (Metric)42 cm
Detector TypePhotomultiplier Tube (PMT)
Incubator TemperatureAmbient +3°C to 45°C
InterfaceSerial RS-232C
Light SourceQuartz Halogen Lamp
Plate Type96 and 384-well Plates
Product LineFluoroskan Ascent
Reading OptionsTop and Bottom
ShakingOrbital
Software TypePC Software
Wavelength SelectionFilters
Unit SizeEach

Frequently asked questions (FAQs)

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer perform spectral scans?

No, they cannot perform spectral scans. For scanning applications, see Varioskan LUX Multimode Microplate Reader information here: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer read time-resolved fluorescence (TRF) or time-resolved FRET (TR-FRET) assays?

No, they cannot read time-resolved fluorescence (TRF/TR-FRET). For these applications, see Varioskan LUX Multimode Microplate Reader information here: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html.

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer read FRET assays?

Yes, they can read any type of fluorescence intensity including FRET assays.

Is there a Windows 10 compatible software for my Fluoroskan Ascent Microplate Fluorometer, Fluoroskan Ascent FL Microplate Fluorometer and Luminometer, or Luminoskan Ascent Microplate Luminometer?

If your reader's serial number begins with 374-9 followed by more digits/letters, then you can order an upgrade kit that includes a USB converter kit and SkanIt Software (Cat. No. N17646). If the serial number is 374- followed immediately by any number other than 9, then you must retain an older Windows 7, 32-bit for PC and continue using Ascent 2.6 software, as your reader's firmware is not compatible with SkanIt Software.