Search
Thermo Scientific™
Varioskan™ Flash Multimode Reader
Run demanding research assays with the utmost flexibility using the Thermo Scientific™ Varioskan™ Flash Multimode Reader.
Have Questions?
Change view
| Catalog Number | Includes |
|---|---|
| 5250040 | Bottom Reading |
| 5250500 | LumiSens Option |
| 5250510 | Dispenser Option, 1 mL Syringe, factory fitted |
Catalog number 5250040
Price (CNY)
-
Includes:
Bottom Reading
Replacement products for these discontinued products can be found on the Varioskan™ LUX multimode microplate reader page.
Run demanding research assays with the utmost flexibility using the Thermo Scientific™ Varioskan™ Flash Multimode Reader. This versatile instrument can be used across a wide variety of applications. It offers unlimited wavelength selection, up to three onboard dispensers and excellent optical performance. The Varioskan Flash Multimode Reader has passed the DLReady™ validation at Promega Corporation™ and is certified as DLReady. Additionally, the reader has been granted the LanthaScreen™ Certified designation by Invitrogen™, and is validated for Thermo Scientific Pierce™ Luciferase Assays.
Varioskan Flash spectral scanning multimode reader includes fluorescence intensity, time-resolved fluorescence (TRF), photometric, and optional luminometric detection technologies. It is designed for optimization and analysis of various assays, such as binding assays, ADMETox, molecular biology assays, enzyme kinetic studies, ion-channel and cell signalling assays.
Features:
Features:
- Unlimited wavelength selection for spectral analysis and measurement at any single wavelength
- Easy measurement setup with automated internal functions ensuring maximal sensitivity and full dynamic
- Onboard dispensers for exact follow-up of kinetic reactions
- Increased assay throughput—reads up to 1536-well plates and can easily be integrated with automated systems
- High performance incubator for temperature critical assays
- Easy assay setup, flexible data handling and convenient report formatting with Thermo Scientific™ SkanIt™ Software
Compatible with:
The Varioskan Flash is compatible with the new Thermo Scientific™ μDrop plate.
Recommended for:
Apoptosis assays; Ca2+ flux assays; Cell proliferation; Cellular assays; Cytotoxicity and ADMETox; Direct DNA, RNA and protein quantitation; ELISA/FIA/TRF-ELISA assays; Enzyme kinetic studies; Europium assays; FRET assays; TR-FRET assays; BRET assays; GPCR assays; Ion channel assays; Kinase assays; Multilabel assays; Reporter gene assays; Signal transduction; Tryptophan and tyrosine UV fluorescence
Specifications
Accuracy (Photometric)±2% or 0.003 Abs, whichever is greater, at 200 to 399 nm (0 to 2 Abs), ±1% or 0.003 Abs, whichever is greater, at 400 to 1000 nm (0 to 3 Abs)
Depth (English)22.8 in.
Depth (Metric)58 cm
DescriptionVarioskan Flash fluorescence intensity (top and bottom reading), photometric and time-resolved fluorescence measurement capabilities. (For luminometric reading capability Varioskan LumiSens option is required)
Dynamic RangeFluorescence intensity, top reading: >6 decades; bottom reading: >5.5 decades
Time-resolved fluorescence, top reading: >6 decades
Time-resolved fluorescence, top reading: >6 decades
Emission Wavelength RangeFluorometry: 270 to 840 nm
Excitation Wavelength RangeFluorometry: 200 to 1000 nm
Height (English)19.7 in.
Height (Metric)50 cm
IncludesBottom Reading
Linearity96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm
Measurement Speed96 wells: 15 sec., 384 wells: 45 sec., 1536 wells: 135 sec.
Measurement TypeFluorescence Intensity, Time-resolved Fluorescence, Photometry and Optional Luminometry (Varioskan LumiSense option required), all with spectral scanning
No. of DispensersUp to 3 Optional
PrecisionSD <0.001 Abs or CV <0.5%, whichever is greater, at 450 nm (0 to 3 Abs)
Product TypeMultimode Reader
SensitivityFluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate
Fluorescence intensity, bottom reading: <4 fmol fluorescein/well, 384-well plate
Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well plate
Fluorescence intensity, bottom reading: <4 fmol fluorescein/well, 384-well plate
Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well plate
Spectral Scanning Speed<2 sec./well, 400 to 500 nm, 1 flash, 2 nm steps
Wavelength RangePhotometry: 200 to 1000 nm
Weight (English)121 to 148 lb., depending on the configuration
Weight (Metric)55 to 67 kg, depending on the configuration
Width (English)21.3 in.
Width (Metric)54 cm
Incubator TemperatureAmbient +4°C to 45°C
Light SourceXenon Flash Lamp
Plate Type6 to 1536-well plates, Photometry: 6 to 384-well plates
Product LineVarioskan
Reading OptionsTop and Bottom
ShakingOrbital
Software TypeSkanIt Software
Wavelength SelectionDouble monochromaters, Double excitation monochromaters, Double emission monochromators
Unit Size-