UltiMate™ WPS-3000SL/TSL Standard Well Plate Autosamplers

Enjoy wide application flexibility in a cost-effective, dependable autosampler. The Thermo Scientific™ Dionex™ UltiMate™ Standard Well Plate Autosampler is highly versatile in sample format and injection volume. It can rapidly, accurately and precisely inject from 1 to 100μL for conventional LC and up to 62 MPa (9000 psi) for UHPLC applications. The in-line split-loop (flow-through) injection design puts the needle and sample loop directly in the high-pressure fluid path. Combined with an external needle wash option, carryover is essentially eliminated.

Flexible Formats for Low-Flow Applications

The sample rack handles up to three well plates (96 or 384 wells—sealed or unsealed) or sample vial trays in any combination. Run your samples in the same format used for preparation. You can even exchange sample plates during a run. An optional cooler preserves thermolabile samples by chilling to 4°C. The UltiMate 3000 RSLCnano Well Plate Autosampler (WPS-3000TPLRS) is the ideal solution for chromatographers looking to work with the highest injection precision at nano, capillary and micro flow rates in UHPLC applications.

Maximize Throughput

• Three-well plate capacity holds up to 1152 samples
• Large injection range from 10nL to 125μL offers application flexibility
• Stable injection valve pressure extends up to 90MPa
• Thermostatted sample compartment keeps temperature constant from 4 to 45°C or 22°C below ambient

Ensure Accurate Small Sample Performance

• Covered and thermostatted sample tray to protect thermo- and UV-labile samples
• Dual-needle injection principle to minimize sample loss
• Injection precision <0.25% RSD at 5μL (typically <0.15% RSD)
• Low carryover (typically <0.01%)
• Injection Mechanism

Using the dual-needle principle, the vial septum of the well seal is pierced by the stainless steel outer needle. The sampler guides the fused silica capillary sampling needle through the piercing needle down to the bottom of the sample carrier. Sample needle height is easily programmed to match sample vial or well depth. Injections of minute sample volumes with virtually zero sample loss—1μL injection per 1μL sample—are performed using the μL-pickup routine. This routine is of particular importance when injecting protein samples that cannot be diluted without the risk of protein loss or alteration.