CaptureSelect™ C-tagXL RoboColumn™ Pre-packed columns

CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

The CaptureSelect C-tagXL Affinity Matrix in this RoboColumn recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag. CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged E-P-E-A proteins directly from complex source materials in a single step with high purity and yield.

Features of C-tagXL Affinity Matrix include:

• Mild elution, making it suitable for pH-sensitive proteins
• Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
• Excellent scalability
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

• Column size: 200 μL, 1 strip, 8 columns (8-row)
• Column dimension: 5-mm diameter, 10-mm bed height
• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect C-tagXL affinity ligand
• Ligand coupling: epoxide coupling
• Binding capacity: 400 nmol/mL resin depending on flow rate, column height, and contact time
• Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine pH 3.0; Neutral: 20 mM Tris, 2.0 M MgCl₂ pH 7; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris 2 mM S-E-P-E-A
• Formulation buffer: 20% (v/v) ethanol
• Operating pressure: 2 bar (maximum)
• Flow rate: 16–150 cm/hr

RoboColumn is a trademark of Repligen.