Automated μSPE Solid Phase Extraction for GC and LC-MS

Achieve reliable and high-throughput analysis by automating cleanup of QuEChERS extracts using the cartridge based Thermo Scientific™ micro Solid Phase Extraction (μSPE) products. The QuEChERS sample preparation method is used to extract pesticides and organic contaminants from food matrices with subsequent analysis by GC or LC-MS. This μSPE capability, which is available for the Thermo Scientific™ TriPlus™ RSH Autosampler and LC systems.

• Replaces the manual dispersive SPE (dSPE) procedure
• Enables reliable and high-throughput extract cleanup
• Is suitable for a wide variety of food matrices, even those with high lipid contents
• Offers a unified method for GC or LC-MS analysis of hundreds of pesticides and organic contaminants
• Enables scale down of sample volume and solvent usage

μSPE for TriPlus RSH Gas Chromatography Autosampler
Enhancing the automated workflow capability of the TriPlus RSH, the μSPE Cleanup Tool for QuEChERS extracts for the TriPlus RSH Autosampler provides today’s routine laboratories with the productivity they need to succeed. Unlike classic vacuum manifold based SPE, the flow rates applied to the μSPE cartridge can be precisely controlled with a liquid syringe on the TriPlus RSH GC Atosampler, assuring optimum recovery. Low flow rates of ∼2 μL/s in the load and elution steps are used for sharp analyte/matrix separation.

Additional Benefits of the μSPE Clean-up Tool

• Unified cleanup method for all food matrices due to one cartridge type, complete with optimized sorbent bed materials.
• No concentration step thanks to miniaturization at the microliter scale, eliminating routine dilution during the SPE elution step.
• Cleaner extracts due to higher selectivity, resulting in extended instrument uptime.
• Green chemistry as a result of highly reduced solvent consumption.
• Cycle time optimization as preparation of the next sample is performed in parallel with the current chromatographic run, enabling an economical workflow.