SMART Digest™ Trypsin Kits
SMART Digest™ Trypsin Kits
Thermo Scientific™

SMART Digest™ Trypsin Kits

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

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Catalog NumberFormatTypeIncludes
60113-101Soluble TrypsinSoluble Trypsin KitSoluble Trypsin, Buffer, Collection Plate
60109-101Resin in Pre-filled PCR TubesTrypsin KitResin, Collection Plate
60109-101-LPHResin in Pre-filled PCR TubesTrypsin KitLow pH Resin, Collection Plate
60109-103Resin in Pre-filled PCR TubesTrypsin KitResin, Collection Plate, SOLAμ SPE Plate
60109-102Resin in Pre-filled PCR TubesTrypsin KitResin, Collection Plate, Filter Plate
60109-101-BBulk Non-magnetic ResinTrypsin KitResin, Buffer
60109-101-MBBulk Magnetic ResinTrypsin KitResin, Buffer
60109-102-BBulk Non-magnetic ResinTrypsin KitResin, Buffer, Collection Plate, Filter Plate
60109-102-MBBulk Magnetic ResinTrypsin KitResin, Buffer, Collection Plate, Filter Plate
60109-103-BBulk Non-magnetic ResinTrypsin KitResin, Buffer, Collection Plate, SOLAμ SPE Plate
60109-103-MBBulk Magnetic ResinTrypsin KitResin, Buffer, Collection Plate, SOLAμ SPE Plate
60113-101-10PKSoluble TrypsinSoluble Trypsin Kit - 10 Pk.Soluble Trypsin, Buffer, Collection Plate
Catalog number 60113-101
Price (CNY)
12,174.77
Each
Add to cart
Format:
Soluble Trypsin
Type:
Soluble Trypsin Kit
Includes:
Soluble Trypsin, Buffer, Collection Plate
Price (CNY)
12,174.77
Each
Add to cart

Obtain high quality analytical results from protein digests using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

Trypsin Protein Digestion

SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues.

Easy and simple

  • Significantly increased speed of sample processing
  • Simple process
  • Easily automate entire protocol for high-throughput processing

Reliable Results

  • Increased reproducibility over existing protocols
  • Higher sensitivity
  • Higher data confidence

Significantly Reduces Sample Preparation Time

Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.

Cleaner Samples

Reduction in the number of chemicals used and the immobilized trypsin design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. This results in results which are easier to interpret.

Couple with SOLAμ SPE Plates for Desalting

When used with the SMART Digest kits for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.

Specifications
DescriptionSMART Digest Trypsin Kit, Soluble
For Use With (Application)Peptide Mapping
IncludesSoluble Trypsin, Buffer, Collection Plate
CaptureNone
CleavageC-terminal side of lysine and arginine amino acids
EnzymeTrypsin
FormatSoluble Trypsin
Product LineSMART Digest
TypeSoluble Trypsin Kit
Unit SizeEach

Frequently asked questions (FAQs)

Can I use the SMART Digest Soluble Trypsin Kit with filter-aided sample preparation (FASP)?

Yes, we recommend the following protocol, which can be optimized as needed:
1. Rinse a Fisher Scientific Vivacon 500, 30kDa MWCO filter (Cat. No. 14558347) to remove trace amounts of glycerin using a fill volume of water. Decant the filtrate and collection vessel. The filter is now ready for use. If you do not want to use the Vivacon filter immediately, then store the pre-rinsed device in the refrigerator with water or buffer covering the membrane surface. Do not allow the membrane to dry out.
2. Dilute sample water to a total volume of 150 µL on the filter.
3. Centrifuge 14,000 RPM for 15 minutes and discard the flow-through.
4. Add 150 µL water.
5. Centrifuge 14,000 RPM for 15 minutes and discard the flow-through.
6. Add 200 µL of SMART Digest buffer.
7. Change the collection vial and add 5 µL of the SMART Digest soluble enzyme to the filter.
8. Parafilm the filters to prevent evaporation of sample during digestion. Incubate the units in a heated bath at 70°C for 60 - 90 minutes.
9. Optional: As needed, add DTT (3 µL of 50 mM DTT), heat at 37°C and incubate for 30 minutes. Alkylate as needed (using 3 µL of 150 mM alkylating reagent).
10. After incubation, add 2 µL of 1% trifluoroacetic acid to the filter. Mix for 1 minute.
11. Centrifuge the filter units at 14,000 RPM for 10 min.
12. As needed, measure protein concentration using a Thermo Scientific NanoDrop Spectrophotometer, or similar procedure.

What is the best way to automate my workflow with the SMART Digest Soluble Trypsin Kit?

To automate the SMART Digest Soluble Trypsin Kit workflow, we recommend using deepwell plates with Sepra Seals, or PCR plates with iron-on seals in combination with an automated liquid handling platform. Any heating unit with a heated lid, capable of providing uniform heating at 70°C may be used.

Can I use chaotropes with the SMART Digest Soluble Trypsin Kit?

The kit is compatible with up to 1M urea, but incompatible with most concentrations of guanidine.

Can I use surfactants with the SMART Digest Soluble Trypsin Kit?

The kit is compatible with many surfactants including OGS, CHAPS, Nonidet, and NP-40.

Does digestion at high temperatures using the SMART Digest Soluble Trypsin Kit result in an increase in chemical modifications?

Thus far, studies have shown that by accelerating the reaction and minimizing the reaction time, fewer chemical modifications are observed in comparison to protocols utilizing elongated digestion times at lower temperatures. For more details, refer to the application note titled 'SMART Digest Compared to Classic In-Solution Digestion of Rituximab for In-Depth Peptide Mapping Characterization'.

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