CaptureSelect™ KappaXP GoPure™ Column

The pre-packed column contains CaptureSelect KappaXP Affinity Matrix, which enables the purification of human immunoglobulins and Fab fragments containing the constant domain of the human kappa light chain.

Features of this affinity matrix include:

• Single-step purification of immunogloblulins, Fab, and Fab2 fragments directly from complex source materials
• Mild elution for Fab fragments and antibodies
• Elution possible at pH 6.0 with 1.5 M MgCl₂
• Excellent pH stability up to 100 mM NaOH for limited exposure
• Specific for human antibodies, works in the presence of serum (bovine/horse)
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

Matrix: agarose-based, epoxide-activated

Average particle size: 65 ± 10 μm

Ligand: CaptureSelect KappaXP affinity ligand

Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin, depending on residence time

IgG binding capacity: 35–45 g IgG/liter resin, depending on residence time

Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl₂, pH 6

Column dimensions: 7 x 25 mm (1 mL column); 16 x 25 mm (5 mL column)

Max. recommended flow: 5 mL/min. (1 mL column); 10 mL/min. (5 mL column)

Max. operation pressure: 2 bar

Formulation buffer: 20% (v/v) ethanol