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Thermo Scientific™
Mammalian beta-Galactosidase Assay Kit
The Thermo Scientific Mammalian β-Galactosidase Assay Kit provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-GalRead more
| Catalog Number | Quantity |
|---|---|
| 75707 | 75 mL |
Catalog number 75707
Price (CNY)
4,746.00
Each
Quantity:
75 mL
Price (CNY)
4,746.00
Each
The Thermo Scientific Mammalian β-Galactosidase Assay Kit provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.Choose stand-alone assay reagent or complete kit for cell lysis and detection Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis Also compatiible with prepared lysates from other species such as bacteria
Features of the Mammalian β-Galactosidase Assay Kit:
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Related Products
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce Mammalian beta-Galactosidase Assay Kit
Detection MethodReporter Enzyme
Dye TypeEnzyme Labeled
Format96-well Plate
Quantity75 mL
SubstrateProprietary
Substrate TypeBeta-Gal Substrate
Sufficient For250 microplate wells of adherent cells
TargetBeta-Galactosidase
For Use With (Application)Absorbance
For Use With (Equipment)Microplate Reader
Product LinePierce
Product TypeBeta-Galactosidase Assay Kit
Unit SizeEach
Contents & Storage
Sufficient For: 250 microplate wells of adherent cellsPierce beta-Galactosidase Assay Reagent, 25 mL M-PER Mammalian Protein Extraction Reagent, 25 mL Stop Solution, 25 mL
Store the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.
Store the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.