COMBINATORIAL LIB 1KB CLONED

For GeneArt mutagenesis services, we will need the DNA or amino acid sequence of the gene you would like to mutagenize, the host organism you plan to use (this is important for gene optimization if you choose to include it with your request), the restriction sites you need at the 5'/3' ends and/or to avoid internally, and whether or not you want any other added motifs (e.g., Kozak sequence, stop codons, etc.).

Three criteria are important for degenerate libraries and are quality-controlled in all GeneArt degenerate libraries (excluding the GeneArt Strings DNA Libraries):

- Maximum sequence integrity of the non-degenerate parts
- Maximum sequence variation of the degenerate positions with the requested nucleotide distribution
- Maximum library diversity

Here are some examples:

- Increase or adjust promoter strength or specificity
- Enhance or modulate protein stability
- Modify or combine enzyme properties
- Increase binding affinities of receptors, ligands, and antibodies
- Optimize or alter signal peptide efficiencies
- Destroy protein function while retaining immunogenicity
- Combine and select natural polymorphisms
- Increase protein half life
- Adjust thermal stability

Two standard options are available:
- As a set of linear DNA fragments, ready for restriction enzyme digestion and subcloning.
- Subcloned into any vector and delivered as a glycerol stock and plasmid preparation.

Yes. For this purpose, please send in your own customized vector or a commercially available vector.