eBioscience™ BrdU Staining Kit for Flow Cytometry FITC

The eBioscience BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cellsRead more

Catalog Number	Quantity
8811-6600-42	100 Tests

Catalog number 8811-6600-42

Price (CNY)

7,265.00

Add to cart

Quantity:

100 Tests

Price (CNY)

7,265.00

Add to cart

The eBioscience BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.

This kit is optimized to achieve brighter staining compared to traditional protocols.

Reactivity/Species
Bovine, Canine, Cow, Human, Monkey, Mouse, Non-human primate, Rat, Rhesus

Conjugate
FITC

Laser
Blue Laser

Emit
520 nm

Excite
488 nm

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

FormatKit

Quantity100 Tests

For Use With (Application)BrdU Staining

For Use With (Equipment)Flow Cytometer

Product LineeBioscience

Product TypeStaining Kit

Unit SizeEach

Frequently asked questions (FAQs)

Can I also stain for intracellular targets such as transcription factors and cytokines using the eBioscience BrdU staining kit?

Yes, primary antibodies against additional intracellular targets can be added alongside the Anti-BrdU antibody. However, it is critical that you empirically determine whether these additional antibodies are compatible with this staining protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the BrdU in the eBioscience BrdU staining kit supplied as a sterile formulation for in vivo use?

Yes, the BrdU is supplied as a sterile liquid. The vial should be opened under sterile conditions if it will be used in vivo.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I keep the diluted (1 mM) BrdU in the eBioscience BrdU staining kit at 4 degrees C? Can I refreeze it after use?

The stock (32.5 mM) BrdU can be frozen and thawed up to three times. Furthermore, the stock BrdU can be kept at 4 degrees C for up to one week. However, the diluted (1 mM) BrdU should be used immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Have the BrdU Staining kits been tested on proliferating cells of different species?

We have used this kit to detect proliferating human and mouse cells. Other species have yet to be tested but feline, non-human primate and canine should also work.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the stopping points during flow cytometric analysis when using a BrdU staining kit?

Samples can be stored at 4 degrees C for up to 16 hours (i.e., overnight) after fixation with BrdU Staining Buffer (with or without washing in Flow Cytometry Staining Buffer) with no adverse effect on performance.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all eBioscience™ BrdU Staining Kit for Flow Cytometry FITC FAQs