Pierce™ Glycoprotein Isolation Kit, ConA
Pierce™ Glycoprotein Isolation Kit, ConA
Citations & References (3)
Thermo Scientific™

Pierce™ Glycoprotein Isolation Kit, ConA

The Thermo Scientific Pierce Glycoprotein Isolation Kit (Concanavilin A) allows isolation of glycoproteins from complex mixtures, including serum, tissue andRead more
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Catalog NumberQuantity
8980410 reactions kit
Catalog number 89804
Price (CNY)
6,496.00
Each
Add to cart
Quantity:
10 reactions kit
Request bulk or custom format
Price (CNY)
6,496.00
Each
Add to cart
The Thermo Scientific Pierce Glycoprotein Isolation Kit (Concanavilin A) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing alpha-lined mannose or terminal glucose residues. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Features of this Glycoprotein Isolation Kit:

• High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins
• Fast—glycoprotein purification in less than an hour
• Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate.
• Robust—lectin does not leach from resin when processing sample
• Convenient—complete kit offering lectin resins and spin columns with all necessary reagents
• Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Related Products
Pierce™ Glycoprotein Isolation Kit, WGA
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeAffinity, Agarose Resin
DescriptionPierce Glycoprotein Isolation Kit, ConA
FormatKit, Microcentrifuge, Spin Column
Quantity10 reactions kit
Stationary PhaseLectin
Sufficient For10 Reactions
TargetGlycoprotein
Product LinePierce
TypeGlycoprotein Isolation Kit
Unit SizeEach
Contents & Storage
Sufficient For: Isolating glycoproteins with strong affinity for ConA from 10 samples of up to 640 μL (1 to 1.5 mg total protein) each
• ConA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubes

Store at 4°C.

Frequently asked questions (FAQs)

What is the difference between your Pierce Glycoprotein Isolation Kit, ConA and WGA?

The ConA kit (Cat. No. 89804), which has concanavalin A immobilized on the resin, recognizes alpha-linked mannose, and to a lesser extent, terminal glucose residues. The WGA kit (Cat. No. 89805), which has wheat germ agglutinin immobilized on the resin, recognizes N-acetyl glucosamine (GlcNAc) groups and sialic acid.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I isolate my proteins based on their post-translational modification (PTM) or active binding site?

Enrichment of specific proteins or protein complexes can most easily be accomplished by using target-specific immobilized antibody or PTM specific affinity binding. We offer several kits targeting phosphorylated proteins, alpha- linked mannose and terminal glucose residues, N-acetyl glucosamine, polyubiquinated proteins, ATP binding sites, and active serine hydrolase enzymes. Choose the right protein enrichment kit for your application using the following link (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-purification/protein-enrichment.html).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (3)

Citations & References
Abstract
Lectin affinity as an approach to the proteomic analysis of membrane glycoproteins.
Authors:Ghosh D, Krokhin O, Antonovici M, Ens W, Standing KG, Beavis RC, Wilkins JA
Journal:J Proteome Res
PubMed ID:15359739
The aim was to determine the proportion of membrane glycoproteins captured using concanavalin A or wheat germ agglutinin lectin affinity chromatography. Digests of the isolated proteins were separated by reversed-phase liquid chromatography and analyzed by matrix-assisted laser desorption tandem mass spectrometry. The two lectins identified different groups of proteins with ... More
GlycoSuiteDB: a new curated relational database of glycoprotein glycan structures and their biological sources.
Authors:Cooper CA, Harrison MJ, Wilkins MR, Packer NH
Journal:Nucleic Acids Res
PubMed ID:11125129
GlycoSuiteDB is a relational database that curates information from the scientific literature on glyco-protein derived glycan structures, their biological sources, the references in which the glycan was described and the methods used to determine the glycan structure. To date, the database includes most published O:-linked oligosaccharides from the last 50 ... More
Development of serum glycoproteomic profiling technique; simultaneous identification of glycosylation sites and site-specific quantification of glycan structure changes.
Authors:Ueda K, Takami S, Saichi N, Daigo Y, Ishikawa N, Kohno N, Katsumata M, Yamane A, Ota M, Sato TA, Nakamura Y, Nakagawa H
Journal:Mol Cell Proteomics
PubMed ID:20811073
Characterization and interpretation of disease-associated alterations of protein glycosylation are the central aims of the emerging glycoproteomics projects, which are expected to lead to more sensitive and specific diagnosis and improve therapeutic outcomes for various diseases. Here we report a new approach to identify carbohydrate-targeting serum biomarkers, termed isotopic glycosidase ... More