GeneChip™ WT Terminal Labeling Kit
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Applied Biosystems™

GeneChip™ WT Terminal Labeling Kit

GeneChip™ WT Sense Target Labeling and Control Reagents (900652) and GeneChip WT cDNA Synthesis and Amplification Kits (900672 and 900673)Read more
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Catalog NumberNo. of Reactions
90067130 Reactions
90067010 Reactions
Catalog number 900671
Price (CNY)
1,000,000,000.00
Each
Add to cart
No. of Reactions:
30 Reactions
Price (CNY)
1,000,000,000.00
Each
Add to cart
GeneChip™ WT Sense Target Labeling and Control Reagents (900652) and GeneChip WT cDNA Synthesis and Amplification Kits (900672 and 900673) are being discontinued in June 2010.

The Whole Transcript (WT) Sense Target Labeling Assay has an updated protocol. The GeneChip WT (Whole Transcript) Terminal Labeling Kit is optimized to be used for the fragmentation and labeling steps of the GeneChip WT Sense Target (ST) Labeling Assay.

The WT Terminal Labeling Kit employs a strategy for reproducible DNA fragmentation with a combination of uracil DNA glycosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE 1). The typical input material is random-primed, single-stranded DNA

Following fragmentation, the resulting fragmented DNA can then be labeled using terminal deoxynucleotidyl transferase (TdT) in the presence of a proprietary biotinylated compound, GeneChip DNA Labeling Reagent. Both components are also provided in the WT Terminal Labeling Kit. The final labeled DNA target is ready to be added to the cocktail for hybridizing to the arrays such as the GeneChip Human Exon 1.0 ST Array.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Includes Label or DyeDirect Labeling
No. of Reactions30 Reactions
Product LineGeneChip™
Product TypeWT Terminal Labeling Kit
Quantity30 reactions
Detection MethodBiotin-based
Final Product TypeDNA (Fragmented, Labeled)
FormatKit
Labeling TargetRandom-primed DNA (Single-stranded)
Unit SizeEach

Frequently asked questions (FAQs)

How long does it take to scan an array?

It takes approximately 35 minutes to scan each Exon Array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I use the WT Sense Target Labeling Assay protocol for prokaryotic arrays?

This has not been tested at the moment; therefore, it is not recommended to use the protocol for any application other than on the Exon Arrays.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the typical cRNA yield after the IVT reaction in the first cycle?

Starting with 1 µg of total RNA, following the standard protocol, ≥ 16 µg of cRNA is routinely obtained. Starting with 100 ng of total RNA without the RiboMinus rRNA removal step, typically, 20 to 60 µg of cRNA can be generated after cRNA cleanup.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why do you choose to use an rRNA-reduction strategy but not a poly-A mRNA-specific selection protocol?

It has been shown that a portion of the transcripts in total RNA do not necessarily contain Poly-A tails; therefore, they will be excluded by a poly-A RNA positive selection technique. The rRNA reduction approach will also make the protocol more robust in handling smaller amounts of total RNA samples as little as 1 µg.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why is Betaine added to the RiboMinus Hybridization Buffer?

Betaine increases the hybridization stringency. It equalizes the GC Tm and AT Tm, so the background, non-specific, non-rRNA hybridization to the RiboMinus probes due to high GC content may be reduced.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.