GeneChip™ HT RG-230 PM Array Plate
GeneChip™ HT RG-230 PM Array Plate
Applied Biosystems™

GeneChip™ HT RG-230 PM Array Plate

Description: Proven performance from the industry standard The GeneChip™ HT RG-230 PM 24-Array Plate enables high-throughput expression profiling of multipleRead more
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Catalog NumberQuantity
9012591 x 24-array plate
Catalog number 901259
Price (CNY)
-
Quantity:
1 x 24-array plate
Description: Proven performance from the industry standard
The GeneChip™ HT RG-230 PM 24-Array Plate enables high-throughput expression profiling of multiple samples using the same industry-standard content as the GeneChip Rat Genome 230 2.0 Array.*

The HT RG-230 PM Array Plate enables you to:
• Measure gene expression of more than 30,000 transcripts and variants from more than 28,000 well-substantiated rat genes on a single array
• Get accurate and reproducible data by using multiple independent measurements for each transcript

Key Benefits
• Enables increased productivity and efficiency through parallel processing
   - Process 16, 24, or 96 samples on a single array plate
• Same industry-leading performance and content as the Rat Genome 230 2.0 Array in cartridge format
   - Strong signal and correlation to previous designs
• Multiple independent measurements per transcript for increased confidence in your results
   - Interrogates more sequence than a single probe measurement
•Conveniently packaged
   - Individual 16-, 24-, and 96-array plates available
   - All processing trays included

Content Profile
Sequences used in the design of the array were selected from GenBank™ dbEST and RefSeq. The sequence clusters were created from the UniGene database (Build 107, June 2002) and refined by analysis and comparison with the publicly available draft assembly of the mouse genome from the Whitehead Institute Center for Genome Research (MGSC, April 2002).

*The HT RG-230 PM Array Plate only retains perfect match (PM) probes from the cartridge design. Mismatch (MM) probes were removed.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineGeneChip™
Quantity1 x 24-array plate
TypeHT RG-230 PM Array Plate
ArrayTranscriptome Profiling
Format24-array Plate
Number of Arrays24 arrays
SpeciesRat
Unit SizeEach

Frequently asked questions (FAQs)

Is the hybridization mix for cartridges the same as the hybridization mix for PEG arrays (array plates)?

The hybridization mix for PEG arrays is different from the hybridization mix for cartridges. The concentrations of the hybridization mix are different and in addition, DMSO is not added into the hybridization mix for PEG arrays because it can affect the glue that holds the PEG to the plate.

How long does it take to scan the plate?

96 arrays = 4-4.6 hours
24 arrays = 1.5-2 hours

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

If I have cartridges, can I move to PM only arrays in the middle of my project?

No, continue to finish products using the same procedures and products. New projects can be moved to PM only arrays.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What QC metrics will I use to assess the quality of my data?

Mean absolute relative log expression: MA (RLE)
-Detection of 20X spikes, Bio B, Bio C, Bio D & Cre
-House keeping controls
-Poly A controls
-Antigenomic GC - 12 background probes
-PM mean

Please refer to the QC Metrics for Human, Mouse and Rat HT PM Array Plates QRC for more information (http://tools.thermofisher.com/content/sfs/manuals/ hmr_qc_metrics_qrc.pdf).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Which algorithm do I use to run the analysis for these new HT PM Plate Arrays?

We recommend running the RMA algorithm in the Transcriptome Analysis Console (TAC).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.