CytoScan™ HD Training Kit
CytoScan™ HD Training Kit
Applied Biosystems™

CytoScan™ HD Training Kit

The CytoScan™ HD Training Kit contains arrays and reagents for 24 reactions plus training materials for the complete CytoScan HDRead more
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9018341 kit
Catalog number 901834
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The CytoScan™ HD Training Kit contains arrays and reagents for 24 reactions plus training materials for the complete CytoScan HD Cytogenetics Solution.

Customer training is required for the CytoScan HD Cytogenetics Solution. Different training programs are available based upon your experience level. Please contact your local distributor for details.

CytoScan HD Cytogenetics Solution
The complete CytoScan HD Cytogenetics Solution includes CytoScan HD Array, CytoScan Reagent Kit, easy-to-use Chromosome Analysis Suite (ChAS) Software, and the proven GeneChip Instrument System.

Highest genomic coverage: relevant today and in the future
CytoScan HD Solution provides the broadest coverage and highest performance for detecting human chromosomal aberrations. CytoScan HD Array has greater than 99% sensitivity and can reliably detect 25-50 kb copy number changes across the genome at high specificity with SNP (allelic) call corroboration. With more than 2.6 million copy number markers, CytoScan Array has been recognized for its industry-leading coverage of all OMIM™ and RefSeq genes. On a single platform, CytoScan HD Solution offers high-density resolution of the entire genome, extending throughout promoter and miRNA regions, for relevant aberration detection and reporting. Surpassing the limitations of current database curations, the whole-genome array ensures that novel findings can be catalogued for future discoveries and annotations which are often missed by targeted designs.

High-density SNPs: gene-level heterozygosity determination
CytoScan HD Array includes 750,000 SNPs with over 99% accuracy to detect accurate breakpoint estimation, loss of heterozygosity (LOH) determination, regions identical-by-descent, maternal contamination, and low-level mosaicism. The allelic information provides a powerful visualization of added confidence behind CNV calls as well as information on sample heterogeneity, clonal diversity, and uniparental disomy (UPD).

Support the broadest range of sample types – including FFPE!
CytoScan HD Array supports several different sample types for cancer and constitutional cytogenetic research analysis, including blood, bone marrow, buccal, saliva, fresh and frozen tissues, direct/cultured cells, amniocytes, and FFPE samples.

Optimized reagent kit – streamLined workflow
CytoScan HD Solution has been optimized to save laboratory time, money and resources. The simplified and streamLined assay protocol is aligned with laboratory workflow requirements, making it easier to obtain consistency, high-quality results, and reduce operator error. The reagent kit includes all reagents required for the array, including magnetic beads, except ethanol and a DNA Amplification Kit (Please contact Technical Support for a recommendation on our DNA Amplification Kits). Each component passes strict quality control inspection by us to ensure the highest reproducibility and performance with CytoScan HD Arrays. The CytoScan protocol can be accomplished in 2.5 days with just 7 hours of hands-on time.

Affymetrix NIMBUS Target Preparation Instrument – automate your workflow
NIMBUS Target Preparation Instrument offers your lab the premier in compact liquid-handling instrumentation. The sleek NIMBUS workstation is optimized to automate the post-PCR portion of the CytoScan assay, including the DNA purification and pooling stages. CytoScan Automated Target Preparation Solution is ideal for labs looking to handle 24 or 48 samples with ease and increase sample throughput.

Chromosome Analysis Suite (ChAS) Software: designed by cytogeneticists for cytogeneticists
The popular ChAS Software is tailored to cytogenetic research analysis and reporting. The software is available free of charge as part of the complete CytoScan HD Solution.

Related Links
CytoScan™ HD Array Kit and Reagent Kit Bundle
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Number of Markers2.4 million markers
Quantity1 kit
TypeHD Training Kit
ArrayCytogenetics, Copy Number
FormatGenechip Probe Array
Number of Arrays24 arrays
Unit SizeEach

Frequently asked questions (FAQs)

Which thermocycler can I use for the CytoScan HD assay?

Here is the link to the CytoScan assay user guide: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/703038_cytoscan_assay_UG.pdf
In Appendix D (starting on page 67), the manual outlines specifications for compatible thermocyclers. Any thermocycler that fits those specifications will work.

Note: Use the silver or gold-plated block only, do not use an aluminum block. In order to determine which block you have, look at the serial number on the block. An "A" in the serial number indicates that the block is aluminum; an "S" indicates that it is silver. Also, an aluminum block has a honeycomb appearance between the wells, whereas a silver block is smooth.

What are the recommended genomic DNA extraction methods for CytoScan HD assay?

Methods that include boiling or strong denaturants are not acceptable, because the DNA would be rendered single-stranded. For genomic DNA extraction, we recommend using either the Qiagen Gentra Puregene Kit or the 5 Prime PerfectPure DNA Blood Kit.

Our CytoScan HD Assay requires genomic DNA concentration ≥50 ng/µL. Therefore, the elution volumes for each of the kits will need to be adjusted accordingly to achieve the desired concentration.

The presence of RNA and free nucleotides can interfere with some quantitation methods using a spectrophotometer or a NanoDrop instrument. To eliminate RNA contamination, perform RNase treatment during extraction as follows:
- For QIAGEN Gentra Puregene Kit: Perform RNase treatment as recommended in the extraction kit manual prior to elution of genomic DNA.
- For 5 PRIME PerfectPure DNA Blood Kit: Use only RNase-treated purification columns for extraction of genomic DNA.

The purified genomic DNA extracted using the two methods above should meet the DNA quality specifications per the manufacturer’s kit extraction manual.
Note: Both blood and cell line sample sources have been tested for the CytoScan HD assay.

What are the specifications for the CytoScan HD Array?

The CytoScan HD Array includes 750,000 "genotype-able" SNPs and 1.9 million non-polymorphic probes. The CytoScan HD Array covers both constitutional and cancer genes with: - ISCA constitutional coverage at 1 marker /384 bases
- Complete cancer gene coverage at 1 marker / 553 bases
- 12,000 OMIM genes at 1 marker / 659 bases
- >36,000 RefSeq genes with 1 marker / 880 bases
- Backbone (non-gene) coverage of 1 marker / 1,737 bases across genome for breakpoints

Array format: 49
Feature size: 5 µM
Time to scan per array: ~32 mins
Hyb temperature: 50 degrees C

The CytoScan HD Array uses the following QC metrics:
- SNPQC ≥15
- MAPD ≤0.25
- Waviness SD ≤0.12

I accidentally froze the wash buffers in my CytoScan HD Kit. Can I still use them for purification?

The Purification Wash Buffer and Elution Buffer are not affected by being frozen at -20 degrees C and are safe to use for purification.

How is gender determined by the Cytoscan HD array?

A subset of Y probes is used and run through a Hidden Markov Model to determine gender. Copy number of 0 or 1. 0 indicates female and 1 indicates male.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.