GeneChip™ Human Genome U133 Plus 2.0 Assay
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Applied Biosystems™

GeneChip™ Human Genome U133 Plus 2.0 Assay

The GeneChip™ Human Genome U133 Plus 2.0 Assay is used with the GeneChip Human Genome U133 Plus 2.0 Array.First andRead more
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Catalog NumberNumber of Arrays
90248210 arrays
Catalog number 902482
Price (CNY)
-
Number of Arrays:
10 arrays
The GeneChip™ Human Genome U133 Plus 2.0 Assay is used with the GeneChip Human Genome U133 Plus 2.0 Array.

First and most comprehensive whole human genome expression array:
• Convenient one-array view
• Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts
• Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform
• GeneChip System: Richer data, sharper insights, better decisions

Array Profile
All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. The sequences from which these probe sets were derived were selected from GenBank™, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

In addition, there are 9,921 probe sets representing approximately 6,500 genes. These gene sequences were selected from GenBank, dbEST, and RefSeq. Sequence clusters were created from the UniGene database (Build 159, January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Microarray Analysis
Product LineGeneChip™
Quantity10 reactions
ArrayTranscriptome Profiling
FormatArray Cartridge
Number of Arrays10 arrays
SpeciesHuman
Unit SizeEach

Frequently asked questions (FAQs)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How is this array design principle different from the GeneChip Human Genome U133 type of arrays?

The GeneChip Exon Arrays represent a new array design philosophy for the most comprehensive and informative coverage with the exon as the basic unit of expression analysis. Such an inclusive design enables discovery of new and novel splicing events not previously observed experimentally. Some of the key differences are summarized below using the human arrays as an example; refer to the “GeneChip Exon Array Design” Technical Note and the “Design and Performance of the GeneChip Human Genome U133 Plus 2.0 and Human Genome U133A 2.0 Array” Technical Note for more details.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

On average, how many probes are used to represent a corresponding GeneChip Human Genome U133 (HG-U133) Array probe set where there are 11 probe pairs?

The majority of the HG-U133 Plus 2.0 Array consensus sequences are well covered in the new design with approximately 75% of them containing over 8 probes on the Exon Array. The remaining 25% have less overlap with the new exon probes, and are mostly attributed to EST-based HG-U133 Array probe sets that do not contain an annotated coding region. As a result, in many such cases, the entire HG-U133 Array EST-based consensus sequence is designated as one PSR, with around 4 probes selected for that entire region.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.