GeneChip™ Rat Genome 230 2.0 Assay

The GeneChip™ Rat Genome 230 2.0 Assay is a powerful tool for toxicology, neurobiology, and other applications using rat as a model organism.

• Provides comprehensive coverage of the transcribed rat genome on a single array
• Comprised of more than 31,000 probe sets, analyzing over 30,000 transcripts and variants from over 28,000 well-substantiated rat genes
• Sophisticated bioinformatics tools available through the NetAffx™ Analysis Center to rapidly obtain biologically meaningful results
• Publicly available drafts of the rat genome and leading public rat databases were used to refine sequences and provide a higher quality of data output

Assay Profile
All probe sets represented on the GeneChip Rat Expression Set 230 are included on the GeneChip Rat Genome 230 2.0 Assay. Sequences used in the design of the GeneChip Rat Genome 230 2.0 Assay were selected from GenBank™, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 99, June 2002) and then refined by analysis and comparison with the publicly available draft assembly of the rat genome from the Baylor College of Medicine Human Genome Sequencing Center (June 2002).

The GeneChip Rat Genome 230 2.0 Assay includes representation of the RefSeq database sequences and probe sets related to sequences and refined EST clusters previously represented on the GeneChip Rat Genome U34 Set.

Oligonucleotide probes complementary to each corresponding sequence are synthesized in situ on the arrays. Eleven pairs of oligonucleotide probes are used to measure the level of transcription of each sequence represented on the GeneChip Rat Genome 230 2.0 Assay.