Primeview™ Human Gene Expression Complete 30 Reaction Kit

The PrimeView Human Gene Expression Array included in this kit enables the measurement of more than 36,000 transcripts and variants per sample, resulting in accurate and reproducible gene expression data obtained via multiple independent measurements per transcript. The array cartridge enables expression profiling using probe sets with an emphasis on established, well annotated content. Sequences used in the design of the array were selected from the RefSeq version 36, UniGene database 219, and full-length human mRNAs from GenBank.

Features of the PrimeView Human Gene Expression Array include:
• Excellent gene expression specificity and reproducibility
• Reliable results with multiple independent measurements per transcript (11 probes per set for well-annotated sequences, 9 probes per set for the remainder)
• Complete coverage of the annotated genome
• Highest transcript coverage in the industry with the most recent annotation; covers all well- annotated genes and transcripts from RefSeq

Array content
• Comprised of more than 530,000 probes covering more than 36,000 transcripts and variants, which represent more than 20,000 genes mapped through RefSeq or via UniGene annotation
• EST and mRNA sequences used in the design were clustered and assembled to create consensus sequences that represent alternative splice forms. Each assembly was then analyzed for orientation and alternative 3' end evidence.
• Content chosen to cover all well-annotated genes and transcripts from RefSeq v36, leveraging all available EST and mRNA evidence that fall into the same clusters, to rigorously detect alternate 3' ends of those well-annotated genes
• >1,000 probe sets represent transcripts that have no official gene symbol in UniGene, but are based on predicted RefSeq sequences and UniGene clusters with good evidence of actual transcription

The included GeneChip Hybridization, Wash, and Stain Kit is used with cartridge-format arrays, following the target preparation steps described for 3' eukaryotic, prokaryotic, and exon assays. For convenience, the GeneChip Hybridization, Wash, and Stain Kit reagents are formulated into the fewest number of individual components possible, reducing preparation and handling steps. Most of the reagents are ready-to-use solutions. The kit includes sufficieint solutions to process 30 arrays on the GeneChip Fluidics Station 400 or 450.