CytoScan™ Optima and Confirmation Kit

The CytoScan Optima and Confirmation Kit contains the arrays and all reagents (including Taq polymerase) required to perform the CytoScan Optima assay. The new kit provides 24 sample reactions and 24 rehybridization reactions to enable you to run confirmations on the positive samples. Results can be obtained in as few as 2.5 days. The protocol also contains stopping points to accommodate your schedule.

CytoScan Optima Suite
Approximately 50% of miscarriages are caused by chromosomal abnormalities. Traditional cytogenetic techniques such as karyotyping and FISH are limited by cell culture failure and usually lack the appropriate sensitivity. CytoScan Optima Suite has been designed with input from the cytogenetics community worldwide and empirically optimized from CytoScan HD Suite. The CytoScan Optima array offers whole-genome coverage for accurate detection of chromosomal abnormalities with increased probe coverage targeting 396 relevant regions for prenatal and miscarriage applications. CytoScan Optima Suite includes arrays, reagents, and easy-to-use data analysis software for a cost-effective analysis of your prenatal and product of conception (POC) samples.
CytoScan Optima Suite provides whole-genome coverage and higher resolution in key genetic regions to enable the detection of the following:
• Whole-chromosome aneuploidies and submicroscopic gains and losses—the POC sample in the figure below shows a 46 MB duplication in blue followed by a submicroscopic 2.2 MB deletion in red
• LOH/AOH—the sample in the figure below shows three confirmed purple LOH/AOH segments of different sizes and locations
• Triploidy and maternal cell contamination—the whole-genome view below shows four lines in the allelic difference track concordant with a triploidy in this first trimester POC sample
• Low-level mosaicism—an increase in the signal in chromosome 2 in the figure below shows a ∼20% mosaic trisomy in this chorionic villi (CVS) sample

Chromosome Analysis Suite
Chromosome Analysis Suite (ChAS) was developed with input from leading experts and enables you to easily view and summarize chromosomal aberrations across the genome (figure below). ChAS also includes:
• A database to store your data
• Trio tool analysis
• Flexible reporting tools
• Updated external annotations
• A built-in reference from CVS, amniocytes, cultured cells, POC, and blood samples

The software is available free of charge as part of CytoScan Optima Suite.

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