StemPro™ Osteogenesis Differentiation Kit

For Complete Differentiation of Human Mesenchymal Stem Cells into Bone CellsStemPro™ Osteogenesis Differentiation Kit has been developed for the osteogenicRead more

Catalog Number	Quantity
A1007201	1 Kit

Catalog number A1007201

Price (CNY)

Quantity:

1 Kit

For Complete Differentiation of Human Mesenchymal Stem Cells into Bone Cells

StemPro™ Osteogenesis Differentiation Kit has been developed for the osteogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the osteogenesis pathway and generate
immature and mature osteocytes. Using StemPro™ Osteogenesis Differentiation Kit in combination with MesenPRO RS™ Medium or StemPro™ MSC SFM provides a standardized culture workflow solution for MSC isolation, expansion and differentiation into mineralized matrix-producing osteocytes.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell TypeStem Cells (Mesenchymal)

Culture EnvironmentCO2

Endotoxin Level≤50 EU/mL, ≤ 50 EU/ml

FormatLiquid

GlucoseLow Glucose

Glucose Concentration1000 mg/L

GlutamineGlutaMAX™-I

HEPES BufferNo HEPES

Inorganic SaltsSodium Phosphate

Phenol Red IndicatorNo Phenol Red

Purity or Quality GradeResearch Grade

Quantity1 Kit

Research CategoryDifferentiation

Serum LevelStandard Serum

Sodium Bicarbonate BufferSodium Bicarbonate

Sodium Pyruvate AdditiveSodium Pyruvate

SpeciesHuman

SupplementsOsteogenesis Supplements

For Use With (Application)Stem Cell Differentiation

Product LineGibco, StemPro

Product TypeOsteogenesis Differentiation Kit

Unit SizeEach

Contents & Storage

This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
• StemPro™ Osteocyte/Chondrocyte Differentiation Basal Medium (100 ml; store in the dark at 2°C to 8°C)
• StemPro™ Osteogenesis Supplement (10 ml; store in the dark at -5°C to -20°C)

The Basal Medium ships at room temperature, while the Supplement ships on dry ice.

Frequently asked questions (FAQs)

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).