AlgiMatrix™ Firming Buffer
Product Image
Gibco™

AlgiMatrix™ Firming Buffer

AlgiMatrix™ Firming Buffer is for use with the AlgiMatrix™ 3D Culture System to facilitate the formation of firm sponges inRead more
Have Questions?
Catalog NumberQuantity
A109150150 mL
Catalog number A1091501
Price (CNY)
379.00
Each
Add to cart
Quantity:
50 mL
Price (CNY)
379.00
Each
Add to cart
AlgiMatrix™ Firming Buffer is for use with the AlgiMatrix™ 3D Culture System to facilitate the formation of firm sponges in the 96 well and 24 well plate formats. This buffer allows the users to adjust the firmness of the sponge to meet specific cell and experimental requirements. Should be ordered as a separate component.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialFirming Buffer
Recommended StorageStore at room temperature, 15-30° C
SterilitySterile
For Use With (Equipment)3D Culture System to facilitate the formation of firm sponges in the 96 well and 24 well plate formats
GradeCell Culture Grade
Physical FormLiquid
Product LineAlgimatrix
Quantity50 mL
Unit SizeEach

Frequently asked questions (FAQs)

How stable is AlgiMatrix Matrix at room temperature without degradation?

AlgiMatrix Matrix is stable for 12 months unopened. Once opened, the entire plate needs to be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can other cell viability reagents be used with AlgiMatrix Matrix?

Other than the Invitrogen alamarBlue Cell Viability Reagent, AlgiMatrix Matrix is also compatible with Invitrogen PrestoBlue Cell Viability Reagent and Invitrogen CyQuant Direct Cell Proliferation Assay. The protocol is almost the same as the 2D cell culture, but it needs the blank AlgiMatrix Matrix as a blank control.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How important is calcium in the AlgiMatrix firming and dissolving buffers?

The firming buffer works by adding calcium to the sponge, making it stiffer. The dissolving buffer works by removing calcium from the sponge, making it softer.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there any assay variability when using AlgiMatrix Matrix?

3D cell culture with AlgiMatrix Matrix approaches a more physiological state in terms of cell growth and behavior, but along with that comes the higher variability typically associated with more complex systems. You may need to do more replicates or more controls.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use AlgiMatrix Matrix for primary cells?

Some primary cells (with the exception of stem cells) need to adhere in order to expand. As cells won't adhere to AlgiMatrix Matrix, these cells can still be cultured, but will tend to differentiate rather than expand. We recommend that you plate at a high cell density for primary cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all AlgiMatrix™ Firming Buffer FAQs