Jump-In™ CHO-K1 Kit
Jump-In™ CHO-K1 Kit

Jump-In™ CHO-K1 Kit

The Jump-In™ CHO-K1 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™Read more
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Catalog NumberQuantity
A141481 kit
Catalog number A14148
Price (CNY)
355,170.00
Each
Add to cart
Quantity:
1 kit
Price (CNY)
355,170.00
Each
Add to cart
The Jump-In™ CHO-K1 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ CHO-K1 cell line, to create an isogenic stable cell line with less effort and in less time than traditional cell engineering methods.

The high retargeting efficiency, made possible by the R4 sites in the CHO-K1 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ CHO-K1 Kit Lets You:

• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs
• Generate multiple cell lines in parallel using the simplified work flow
• Easily access the technology without complicated licenses or restrictions to interpret

Save Time with Rapid and Efficient Generation of Engineered Cell Lines
With the Jump-In™ CHO-K1 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined protocol makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

Expand Your Experimental Capabilities
The Jump-In™ CHO-K1 Kit is the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway™ destination vectors accept large inserts.

The kit includes:
• pJTI™ R4 Dest CMV pA vector (100 μg)
• pJTI™ R4 Int vector (100 μg)
• Jump-In™ CHO-K1 Cells (2 vials @ 1 ml each)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineJump-In™ CHO-K1
Expression SystemMammalian
FormatKit
Key FunctionsStable Cell Line Development, Targeted Integration
Product TypeCHO-K1 Kit
PromoterCMV
Quantity1 kit
Selection Agent (Eukaryotic)Hygromycin
System TypeJump-In™
Product LineJump-In
Unit SizeEach
Contents & Storage
The kit includes:
Jump-In™ CHO-K1 cells
pJTI™ R4 Int (integrase vector)
pJTI™ R4 DEST CMV pA (destination vector)

Upon receipt, immediately store the cells in liquid nitrogen and the vectors at -20 °C. Cells stored at -80°C can quickly lose viability.

Frequently asked questions (FAQs)

I am planning to generate a Jump-In platform cell line. Do you recommend mapping the site of integration and checking against the database to pick a clone where integration has occurred in a "good" hot spot?

We would recommend engineering an expression marker/reporter in the plasmid used to create the platform line, and then screening the platform line for expression of this marker to identify a high-expressing locus. Otherwise, the process can get quite labor-intensive, as multiple lines would have to be screened after retargeting.

Does the pJTI PhiC31 Int vector contain a nuclear localization signal (NLS), and would adding an NLS increase the efficiency of site-specific integration at pseudo attP sites?

The pJTI Phic31 Int vector does not contain an NLS. Adding an NLS could increase the efficiency of site-specific integration at pseudo attP sites, but there are no data to support it. There is one paper describing the use of an NLS on a PhiC31 integrase vector, but the authors didn't measure integration into pseudo attP sites.

In the Jump-In system, how much DNA or what controls do I need to include in order to get one integration event?

The amount of DNA to be used to obtain single copies should be determined by control experiments done in the absence of integrase. The same amount of DNA that yields less than 5 colonies in the absence of integrase should be used in the presence of integrase. Typically, the integrase expression plasmid makes up most of the amount of DNA used for transfection.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

When should I use the Jump-In Fast system versus the Jump-InTI system?

We recommend using the Jump-In Fast system if you need stable mammalian expression and want to quickly generate well-expressing clones. You can have well-expressing clones with one or more integrations at the PhiC31 pseudo-att P sites. A Southern blot is necessary to confirm the number of integrated events. Use the Jump-InTI system if you need isogenic expression, where every cloned gene would be expressed from the same locus in the same background, with no chromosomal position effects.

What is the difference between the Jump-In and Flp-In systems?

The Jump-In system is PhiC31-integrase mediated and is a stable, targeted, and irreversible mammalian expression system. It consists of the Jump-In Fast system that involves a single integration step and the Jump-InTI (targeted integration) system that needs two integration steps, both of which are targeted and irreversible. In contrast, the Flp-In system is a stable, targeted mammalian expression system that is reversible. The first integration is random (integration of pFRT/lacZeo), and the second integration (integration of the Flp-In expression vector) is targeted but reversible.