MEM, high glucose, high sodium bicarbonate
MEM, high glucose, high sodium bicarbonate
MEM, high glucose, high sodium bicarbonate
MEM, high glucose, high sodium bicarbonate
Gibco™

MEM, high glucose, high sodium bicarbonate

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be usedRead more
Have Questions?
Catalog NumberQuantity
A1451801500 mL
Catalog number A1451801
Price (CNY)
488.60
飞享价
Ends: 31-Dec-2025
623.00
Save 134.40 (22%)
Each
Add to cart
Quantity:
500 mL
Customize this product
Price (CNY)
488.60
飞享价
Ends: 31-Dec-2025
623.00
Save 134.40 (22%)
Each
Add to cart

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using our media selector tool.


This MEM is modified as follows:
WithWithout
• Phenol red• L-glutamine
• 6 g/L D-glucose• HEPES
• 3.7 g/L sodium bicarbonate 



Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
For Use With (Application)Mammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeMEM (Minimum Essential Medium)
Quantity500 mL
Regulatory StatusFor Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Bicarbonate
Without AdditivesNo Glutamine, No HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8 Deg;C. Protect from light



Shipping conditions: Ambient



Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM, high glucose, high sodium bicarbonate FAQs