CytoTune™-iPS 2.0 Sendai Reprogramming Kit

Name: CytoTune™-iPS 2.0 Sendai Reprogramming Kit
SKU: A16517

The CytoTune-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune-iPS Sendai Reprogramming Kit.

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Catalog Number	Quantity
A16517	1 Ea.
A16518	3 Pk.

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Catalog number A16517

Price (CNY)

Quantity:

1 Ea.

The CytoTune-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune-iPS Sendai Reprogramming Kit. This system continues to be easy to use and works with many different somatic cell types, using Sendai particles to deliver Yamanaka factors, which have been shown to be critical for efficient generation of iPSCs. This kit includes three vector preparations: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4.

Features include:

Ease of use— still requires only one application of the virus to obtain successful reprogramming
Increased efficiency—usually at least two-fold higher reprogramming efficiencies than the original kit
Rapid clearance—use the non-integrating Sendai virus that remains in the cytoplasm, reducing the likelihood of integration into the host genome. With the CytoTune-iPS 2.0 Sendai virus, the Sendai backbone and transgenes clear the cytoplasm faster than the original.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Delivery TypeSendai virus

For Use With (Application)Stem Cell Engineering & Reprogramming

Product LineCytoTune™

Product TypeSendai Reprogramming Kit

PromoterProprietary

Quantity1 Ea.

Shipping ConditionDry Ice

Sub Cellular LocalizationCytoplasm

Cell TypeiPSC

FormatKit

Unit SizeEach

Contents & Storage

3 Tubes, store (at -68°C to -85°C.)

Frequently asked questions (FAQs)

What is the shelf life of the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517)?

The shelf life for this kit is 3 years from the date of manufacture. Both the date of manufacture and the date of expiration are stated on the lot-specific Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Stem Cell Culture Support Center.

What is the shelf life of the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

The shelf life for the CytoTune-iPS 2.0 Sendai Reprogramming Kit is 2 years when stored at -80 degrees C with no repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the optimal passage number for reprogramming patient fibroblasts using the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

We recommend reprogramming patient cells at the earliest passage possible. However, it is important to have the cells growing and healthy, which can take between 1-4 weeks. The cells are usually ready to reprogram once they have gone through a total of 3-4 passages.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I scale down my CytoTune-iPS 2.0 Sendai reprogramming experiments?

Yes. Initial experiments with fibroblasts have shown that scaling down to a 12-well or 24-well works, but at a potentially reduced efficiency. Cell seeding densities may need to be optimized.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many cells do I need to start my reprogramming experiment with the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

We recommend seeding approximately 50,000-100,000 fibroblast cells per well of a 6-well to yield 200,000-300,000 cells two days later. This equals a confluency of 50-80% on the day of transduction. Overly confluent cells will result in a decreased transduction efficiency. When cells are overly confluent (>80% confluent), we recommend re-plating the cells to achieve a range of 50-80% confluency on the day of transduction.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CytoTune™-iPS 2.0 Sendai Reprogramming Kit FAQs