Amplex™ Red Galactose/Galactose Oxidase Assay Kit

The Amplex™ Red Galactose/Galactose Oxidase Assay Kit provides a sensitive and simple method for detecting galactose oxidase activity or galactose using a fluorescence microplate reader or fluorometer.

See our complete line of Fluorescence Microplate assays.

• Detect concentrations as low as 2 mU/mL of galactose oxidase activity or 4 μM galactose
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference

Galactose oxidase is an important tool in the detection of galactose in biological fluids. The Amplex™ Red Galactose/Galactose Oxidase Assay Kit provides an ultrasensitive method for detecting galactose or for monitoring galactose oxidase activity. Galactose oxidase catalyzes the oxidation of galactose at the C6 position and generates hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts in a 1:1 stoichiometric ratio with the Amplex™ Red reagent to generate the red-fluorescent compound resorufin.

Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.

Use Amplex™ Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex™ Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex™ UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.