Ion Universal Library Quantitation Kit

The Ion Universal Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input generated by the Ion 16STM Metagenomics Kit for Ion Torrent™ semiconductor sequencing. This kit provides the TaqMan™ assay, TaqMan™ Universal Master Mix, and library standard needed to quantify femtomolar amounts of Ion fragment libraries. With cycling conditions that allow both fast and standard cycling, the Ion Universal Library Quantitation Kit supports most real-time platforms. This real-time PCR kit enables greater accuracy and specificity compared to non-PCR or non-probe-based methods, delivering simplicity and speed to the Ion PGM™ System sequencing workflow.

The Ion Universal Library Quantitation Kit enables:

• Precision—TaqMan™ assays enable greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods
• Sensitivity—the protocol supports quantitation of femtomolar amounts of fragment libraries
• Simplicity—the validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation
• Versatility—the highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range
• Efficiency—qPCR-based methods use less material for quantification

This kit includes:

• A TaqMan™ assay designed for Ion library adaptors
• TaqMan™ Universal PCR Master Mix
• An adapted E. coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ∼190 bp in size

Note: In addition to use with the Ion 16S™ Metagenomics Kit workflow, the Ion Universal Library Quantitation kit is also compatible with sequencing workflows for amplicons generated using any master mix.