ExpiCHO-S™ Cells
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ExpiCHO-S™ Cells
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ExpiCHO-S™ Cells

ExpiCHO-S Cells are derived from a non-engineered subclone that has been screened and isolated from CHO-S Chinese hamster ovary (CHO) cells.
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Catalog NumberFormat
A29127
A29132
Catalog number A29127
Price (CNY)
17,480.00
Each
Add to cart
Price (CNY)
17,480.00
Each
Add to cart
ExpiCHO-S Cells are derived from a non-engineered subclone that has been screened and isolated from CHO-S Chinese hamster ovary (CHO) cells (1). They are a core component of the ExpiCHO Expression System Kit (Cat. No. A29133). ExpiCHO-S Cells are maintained in suspension culture, have minimal tendency to clump, and grow to high density in ExpiCHO Expression Medium (Cat. No. A2910001). As part of the ExpiCHO Expression System Kit, the cells generate superior protein yields by transient transfection compared to standard CHO and 293 cells.

Features of ExpiCHO-S Cells include:

  • Suspension culture cells adapted in ExpiCHO Expression Medium
  • Demonstrated yields of up to 3 g of protein per L of transfected culture as part of the ExpiCHO expression system
  • Rapid doubling times of approximately 17 hours
  • Consistent transient expression performance for over 20 passages after thaw
  • High cell viability and productivity for greater than 10 days post-transfection as part of the ExpiCHO expression system
  • Enable rapid, high-yield transient protein production, allowing labs developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells per mL in ExpiCHO Expression Medium and 10% DMSO. The cells should be thawed directly into ExpiCHO Expression Medium. The 6-vial multipack eliminates the need to expand and bank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL up to 5 L when using a Nalgene 5-L angled-bottom flask. Protein harvest can be performed at 5 to 14 days post-transfection, depending on the protein expressed.

Reference

1. Wurm, FM. CHO Quasispecies—Implications for Manufacturing Processes (2013) Processes 1:296-311.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 mL
Unit SizeEach

Frequently asked questions (FAQs)

What is the biosafety level of ExpiCHO-S Cells (Cat. No. A29127)?

ExpiCHO-S cells are biosafety level 1.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

When subculturing ExpiCHO the day before a transfection to 3-4 x 10E6/mL, would you recommend pelleting the cells and resuspending them in completely fresh media, or simply replacing some of the media to split them down to the desired cell density?

Before transfecting ExpiCHO cells, there is no need to change out the media during the subculturing. This would become expensive and the conditioned media is not necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how many passages would you recommend using ExpiCHO cells before replacing them with new cells that were frozen at earlier passages?

For optimal performance with respect to protein production, we recommend thawing ExpiCHO cells and letting them grow for 2-3 passages prior to transfection. Overall, we do not recommend using the cells beyond ˜20 passages.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

With the ExpiCHO Expression System, can I shake faster or slower than recommended?

We have provided optimal shake speeds for various formats. For most flasks, there is a drop-off in expression when you go too fast or slow because of cell shear stress or insufficient aeration. The effect is sharper in plates where there is a sharper transition between static and moving fluid in wells.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How many days should I wait before harvesting my protein with the ExpiCHO system?

Optimal time to harvest protein will depend on the specific properties of the protein being expressed and the protocol chosen. Typical harvest times to reach maximum titers for the various protocols are as follows:
- Standard Protocol: 8-10 days post-transfection
- High Titer Protocol: 10-12 days post-transfection
- Max Titer Protocol: 12-14 days post-transfection

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

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