IonCode™ Barcode Adapters 1-96 Kit
IonCode™ Barcode Adapters 1-96 Kit
Ion Torrent™

IonCode™ Barcode Adapters 1-96 Kit

The IonCode Barcode Adapters 1-96 Kit provides a set of 96 unique barcode adapters specifically designed for optimal performance withRead more
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Catalog NumberQuantity
A29747960 Reactions
Catalog number A29747
Price (CNY)
-
Quantity:
960 Reactions

The IonCode Barcode Adapters 1-96 Kit provides a set of 96 unique barcode adapters specifically designed for optimal performance with the Ion GeneStudio S5, Ion PGM, and Ion Proton semiconductor sequencers. When used in combination with the Ion AmpliSeq Library Kit PLUS or Ion AmpliSeq Library Kit 2.0, this kit enables users to pool up to 96 amplicon libraries and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment.

Features of the IonCode Barcode Adapters 1-96 Kit include:

  • Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes
  • Both sequence- and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
  • Robust error correction built in by design for added confidence in sample identification

Multiplexing with barcode adapters enables higher throughput

Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias.

Sequence- and flow-optimized for increased performance and efficiency

Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool and require minimal number of flows to interrogate, resulting in more economical multiplexed sequencing runs.

Robust error correction built in by design

Built-in error correction prevents the possibility of incorrect sample assignment.

For Research Use Only. Not for use in diagnostic procedures
Specifications
For Use With (Application)For Use with Target Ampliseq DNA and RNA Library Preparation
For Use With (Equipment)Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
FormatKit
Nucleic Acid TypeDNA, RNA
Product TypeBarcode Adapter Kit
Quantity960 Reactions
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
1 x 96-well plate, 20 μL per well

Store in freezer (-5°C to -30°C).

Frequently asked questions (FAQs)

What do I need to do to prep the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reactions are possible with the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

In what format are the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit is shipped on dry ice and we recommend storing at -30 degrees C to -5 degrees C in an upright position.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle (Cat. No. A46497) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, the IonCode Barcode Adapters, the Ion 550 Kit - Chef, and the Ion 550 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 384 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.