Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef (1 sequencing run per initialization)
Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef (1 sequencing run per initialization)
Ion Torrent™

Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef (1 sequencing run per initialization)

The Ion 510 & Ion 520 & Ion 530 Kit-Chef (1 sequencing run per initialization) enables robust and automated templateRead more
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Catalog NumberQuantity
A340198 Reactions
Catalog number A34019
Price (CNY)
-
Quantity:
8 Reactions
The Ion 510 & Ion 520 & Ion 530 Kit-Chef (1 sequencing run per initialization) enables robust and automated template preparation and sequencing of up to 400 base-read libraries using the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system. The Ion 510 & Ion 520 & Ion 530 Kit contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability. This kit accommodates 1 sequencing run per initialization. For 2 sequencing runs per initialization (200 base-read applications only), see Cat. No. A34461.

The Ion 510 & Ion 520 & Ion 530 Kit-Chef workflow:
• Provides template preparation and chip loading reagents for up to 400 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for the Ion 510, Ion 520, and Ion 530 chips

The Ion S5 and Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 510 & Ion 520 & Ion 530 Kit with the Ion 510 Chip Kit (up to 3 million reads per chip), the Ion 520 Chip Kit (up to 5 million reads per chip), or Ion 530 Chip Kit (up to 20 million reads), allowing for flexibility of total throughput. For even greater flexibility, combine different chip types in a single Ion Chef run when using Torrent Suite Software 5.6 and above. For de novo research applications, add the Ion S5 Calibration Standard to prepared libraries for improved base-calling accuracy.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeChef Kit
Quantity8 Reactions
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Throughput400 Base-Read Libraries
Product LineIon Chef™, Ion Torrent™
Software TypeTorrent Suite Software 5.6
Unit SizeEach
Contents & Storage
• Ion S5 Chef supplies, store at room temperature
• Ion S5 Chef solutions, store at room temperature
• Ion 510 and Ion 520 and Ion 530 Chef reagents, store at -5 to -30°C
• Ion S5 Sequencing solutions, store at room temperature
• Ion S5 Sequencing reagents, store at -5 to -30°C

Frequently asked questions (FAQs)

Why did my Ion Chef Instrument stop during a run?

- The instrument may have encountered an error. We recommend recording the error shown on the instrument display, then tapping OK. For further assistance, contact Technical Support at techsupport@thermofisher.com.
- A consumable may have been improperly loaded. We recommend ensuring that all consumables are correctly loaded. For instructions, see the user guide for your templating kit. For further assistance, contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Why is the door of the Ion Chef Instrument not opening?

- An obstruction may be present on or around the door mechanism. We recommend removing the obstruction blocking the door, then resuming normal instrument operation.
- The instument may be experiencing a hardware or software error. We recommend contacting Technical Support at techsupport@thermofisher.com.C8

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Why does the Ion Chef Instrument not display the home screen when powered on?

Multiple causes are possible. We recommend powering off the instrument, waiting for 30 secs and then powering on the instrument. If you continue to experience the issue, contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

For the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay, what are the analysis workflows in Ion Reporter Software?

There are two analysis workflows available:

1. Oncomine TagSeq Lung v2 Liquid Biopsy - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.1% limit of detection), Fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell‑Free Total Nucleic Acid Research Assay. This is compatible with DNA & RNA purified from cell-free total nucleic acids.

2. Oncomine TagSeq Lung v2 Tumor - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.5% limit of detection), fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Due to deamination events caused by the FFPE process, the minimum alternative allele frequency is set to 0.3%. This makes it compatible with DNA & RNA purified from FFPE tumor tissue as well as fresh frozen tumor tissue.

Are there any BED files for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay?

Yes, there is a specific BED file and Hotspot file for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Please contact your local Field Application Specialist (FAS) or Clinical Application Consultant (CAC) to request the BED files.