Ion 550™ Kit—Chef
Ion 550™ Kit—Chef
Ion Torrent™

Ion 550™ Kit—Chef

The Ion 550 Kit enables robust and automated template preparation and sequencing of up to 200 base-read libraries using theRead more
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Catalog NumberQuantity
A345418 reactions
Catalog number A34541
Price (CNY)
-
Quantity:
8 reactions
The Ion 550 Kit enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime or Ion S5 XL sequencing systems.

The Ion 550 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of eight loaded chips with complete run traceability.

The Ion 550 Kit, together with the Ion 550 Single Chip Supplemental Kit, enables the flexibility to prepare one or two loaded chips per Ion Chef run, without loss of templating reagents.

The Ion 550 Chip workflow:
• Provides template preparation and chip loading reagents for up to 200 base–read libraries
• Maximizes flexibility with the ability to load one or two Ion 550 chips per Ion Chef run
• Simplifies your research with ready-to-use, disposable reagent cartridges
• Delivers fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy

The Ion GeneStudio S5 Plus and S5 Prime systems, along with the Ion Chef Instrument, provide reproducible sequencing workflows. Delivering up to 130 million reads in a single run, the Ion 550 Chip empowers rapid exome and transcriptome analysis, as well as high coverage for cell free nucleic acid applications. Combined with Ion AmpliSeq technology and Ion Reporter Software for data interpretation, the Ion 550 Chip workflow provides a cost-effective and comprehensive solution for your inherited disease or oncology research needs.

Specifications
For Use With (Application)Comparative Sequencing (Germline Mutations - 50:50)
For Use With (Equipment)Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime, Ion S5 XL Sequencing System
Product TypeChef Kit
Quantity8 reactions
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Throughput200 Base-Read Libraries
Product LineIon Torrent
Unit SizeEach
Contents & Storage
• 1 Ion 550 Chef Reagents, store at -20°C
• 1 Ion 550 Chef Solutions, store at room temperature
• 4 Ion S5 Chef Supplies, store at room temperature
• 1 Ion S5 Sequencing Solutions, store at room temperature
• 1 Ion S5 Sequencing Reagents, store at -20°C

Frequently asked questions (FAQs)

Is Ion S5 Sequencing Reagents (Part No. A27768) used for both the Ion 540 and Ion 550 Chip sequencing?

Yes, Ion S5 Sequencing Reagents (Part No. A27768) is used on the instrument for both Ion 540 and Ion 550 Chip sequencing.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center

When setting up a single chip run with the Ion 550 Kit-Chef, I get an error message indicating that there is a chip type mismatch. What does it mean?

In most cases, a chip type mismatch error indicates that the Ion Chef S5 Series Chip Balance was placed incorrectly in the Chip-loading centrifuge. Please make sure that the Ion Chef S5 Series Chip Balance is placed in Position 2 of the Chip-loading centrifuge.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How do I ensure cartridge-use tracking with the Ion 550 Kit-Chef?

To use the flexible workflow feature (single chip/dual chip) and enable cartridge-use tracking between Torrent Servers across multiple Ion Chef instruments, Torrent Servers must be linked in an Ion Mesh configuration. For further information on how to link Torrent Servers in an Ion Mesh setup, go to Software Administration -> Set up Ion Mesh in Torrent Suite Software Help.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What version of Torrent Suite Software is compatible with the Ion 550 Kit-Chef?

The Ion 550 Kit-Chef is compatible with Torrent Suite Software version 5.8 and later.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the maximum library read length allowed with the Ion 550 Kit-Chef?

The Ion 550 Kit-Chef can be used with up to 200-base-read libraries of any type, prepared using any available Ion library kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.