ExpiSf9™ Cells

ExpiSf9 Cells are a non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth in ExpiSf CD Medium (Cat. No. A3767802).

ExpiSf9 Cells:

• Enable consistent protein expression performance for over 20 passages after thaw
• Allow you to attain yields of three times more protein per liter than other insect cells from baculovirus-infected culture as part of the ExpiSf Expression System
• Achieve maximum cell densities of 2 x 10⁷ cells per mL in ExpiSf CD Medium
• Exhibit fast recovery post-thaw and doubling times of approximately 24 hours
• Achieve high performance growth in multiple culture volumes from 4 mL in deep-well plates to >3 L in shake flasks

ExpiSf9 Cells are a core component of the ExpiSf Expression System. Frozen cells are supplied in a vial containing 1.5 mL of cells at 1 x 10⁷ viable cells per mL in ExpiSf CD Medium and 7.5% DMSO. The cells should be thawed in ExpiSf CD Medium. Cells are typically grown in shake flasks on a shaker platform in a non-humidified, non-CO₂ cell culture incubator. Growing ExpiSf9 cells in adherent format for routine culture is not recommended.