LV-MAX™ Lentiviral Production System

The Gibco LV-MAX Lentiviral Production System is an optimized, scalable system designed to maximize lentiviral vector production. This system is specifically designed for high-density suspension cultures of HEK293-derived Viral Production Cells, which are adapted to a chemically defined, serum-free, and protein-free LV-MAX Production Medium. The kit also includes a high-efficiency, cationic, lipid-based transfection reagent, LV supplement, and LV enhancer, designed for the transient transfection of high-density suspension-adapted Viral Production Cells. All components work synergistically to generate superior and functional lentiviral particles under serum-free conditions compared to conventional polyethylenimine (PEI).

Key features of the LV-MAX Lentiviral Production System

• High LV yields—achieves functional titers up to 1 x 10⁸ TU/mL (unconcentrated)
• High-density growth—supports growth of Viral Production Cells at densities greater than 1 x 10⁷ cells/mL for maximum vector output
• AOF formulation—chemically defined, serum-free, and animal-origin-free medium ensures consistent performance by eliminating variability from undefined components
• Ready-to-use—no media supplementation required; simplifies workflows and provides reliable, lot-to-lot reproducibility in lentivirus production
• Expression system compatible—fully compatible with the LV-MAX Lentiviral Production System for seamless integration into end-to-end lentivirus manufacturing processes
• Scalable and reproducible transfection and production—validated from 96–well plates to multi‐liter bioreactors, enabling easy scale-up without sacrificing cell yields
• Easy and robust culture and transfection protocols, seamlessly integrating into any transient production workflow

Kit components

• LV-MAX Viral Production Cells (VPC)—HEK293-derived cells adapted to high-density suspension culture, providing a robust platform for lentiviral vector production
• LV-MAX Production Medium—a chemically defined, serum-free, protein-free medium, specially developed for high-density growth and transfection of Gibco Viral Production Cells
• LV-MAX Transfection Reagent—a high-efficiency, cationic, lipid-based reagent uniquely formulated for co-transfecting multiple plasmids into high-density Viral Production Cell cultures. This reagent ensures high transfection efficiency with minimal toxicity.
• LV-MAX Supplement—an optimized, chemically defined, serum-free, protein-free, animal origin-free formulation designed to suppress cell growth during transfection. It enhances lentiviral vector production without compromising cell viability or interfering with the production process.
• LV-MAX Enhancer—a proprietary, animal origin-free formulation that significantly boosts lentiviral vector production

Applications

• Gene therapy—ideal for the production of lentiviral vectors for gene therapy research and development
• Cell therapy—supports the production of lentiviral vectors for cell therapy applications, enabling a seamless transition from discovery to clinical production
• Research and development—provides a reliable and scalable solution for lentiviral vector production in various research settings

Components are available in RUO or CTS

Offered as both a Research Use Only (RUO) version and a GMP-compliant Cell Therapy Systems (CTS) version to support translational and clinical-scale applications.