Ion AmpliSeq™ HD Dual Barcode Kit 1-24

The Ion AmpliSeq HD Dual Barcode Kit 1-24 provides a set of 24 unique dual-barcode primers designed and validated for optimal performance with Ion GeneStudio S5 series sequencers. This barcode kit enables up to 24 samples to be multiplexed on a single chip. The barcode kit is only compatible with the Ion AmpliSeq HD Library Kit (Cat. No. A37694) and should not be used with any other library kit.

Features of the Ion AmpliSeq HD Dual Barcode Kit 1-24 include:

• Enables multiplexing of up to 24 library samples on a single sequencing chip
• Dual barcoding at both the 5' and 3' end for enhanced sample tracking
• Barcodes have been sequence- and flow-optimized for equal representation of all barcodes within a pool
• Provided in a single-use 96-well plate for ease of use

Sample multiplexing

Sample multiplexing using the Ion AmpliSeq HD Dual Barcode Kit 1-24 allows for multiple samples to be run on the same chip, thus decreasing the amount of reagent used on a per sample basis.

Dual-barcoding

Use of dual-barcodes has been shown to provide greater confidence in sample tracking and enhances the ability to detect any cross-barcode events. The barcodes are added at the 5' and 3' ends as part of the library preparation protocol.

Sequence- and flow-optimized

The Ion AmpliSeq HD Dual Barcode Kit 1-24 contains primers with unique sequences that have been optimized to provide equal representation of all barcodes in a pool, with a minimum number of flows. This helps achieve economical multiplexed sequencing runs.

24 single-use dual-barcodes in a 96-well plate format

The dual barcodes come in a sealed 96-well plate. Spacing of the barcodes prevents cross-barcode mixing and enables easy access to individual barcodes. A single reaction is provided in each well, which prevents the contamination challenges faced when using multi-use tubes.