Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL

Perform high-throughput protein recovery and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Pierce Zeba desalting chromatography columns, plates, and cartridges. Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with ≥95% retention of salts and other small molecular contaminants (<1,000 Da).

These Zeba spin desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labelling reagents.

Benefits of Zeba desalting columns, plates, and cartridges include:

• High recovery—low-binding resin maximizes protein recovery,
• Fast—no fraction screening or waiting for protein to emerge by gravity flow
• Economical—great performance at lower cost than other commercially available cartridges
• Easy-to-use—no cumbersome column preparation or equilibration
• Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs 

Zeba Spin Desalting Columns 

Convenient, ready-to-use polypropylene columns are the ideal solution for quick and straightforward desalting and buffer exchange of protein samples in a centrifuge format. The pre-dispensed spin columns are offered in various resin fills (75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL, 20 mL). These options make it simple to handle sample volumes between 2 μL and 10 mL.

Zeba Desalting Plates

Provide trouble-free high-throughput desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. Pre-dispensed 96-well filter plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes. Compatible with centrifugation for manual or automated purification.

Zeba Desalting Chromatography Cartridges

Compatible with automated liquid chromatography systems and with syringe processing, Zeba desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.