Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL
Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL
Thermo Scientific™

Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL

The new and enhanced Zeba Spin and Micro Spin Desalting columns and plates are designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance.
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Catalog NumberQuantitySample Volume (Metric)
A4000247610 Columns4 to 10 mL
A400024715 Columns4 to 10 mL
A5775625 columns5 to 14 μL
A5775850 columns5 to 14 μL
A5775925 columns50 to 150 μL
A5776050 columns50 to 150 μL
A577615 columns300 to 800 μL
A5776225 columns300 to 800 μL
A577635 columns500 to 2000 μL
A5776425 columns500 to 2000 μL
A577655 columns1000 to 4000 μL
A5776625 columns1000 to 4000 μL
A577672 plates20 to 100 μL
A577684 plates20 to 100 μL
Catalog number A40002476
Price (CNY)
4,339.00
10 columns
Add to cart
Quantity:
10 Columns
Sample Volume (Metric):
4 to 10 mL
Price (CNY)
4,339.00
10 columns
Add to cart
Thermo Scientific Zeba spin and micro spin desalting columns and plates are designed for efficient desalting and buffer exchange of protein samples. They contain a proprietary size-exclusion chromatography resin with a robust bead structure that withstands centrifugal force and helps produce better flow characteristics and increased efficiency in removing low molecular weight protein contaminants from samples. The Zeba resin performs well even with significantly diluted protein samples and eliminates over 95% of contaminants without causing protein loss.

These Zeba spin and micro spin desalting columns and plates are designed for desalting and buffer exchange of proteins with a molecular weight >40 kDa and removal of small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance, with expanded capacity to accommodate up to 10 mL of sample volume.

Features of Zeba spin and micro spin desalting columns include:

  • High-performing resin—exceptional protein recovery at lower protein concentrations
  • Fast and efficient—eliminate sample dilution, fraction collection, and the need for expensive chromatography systems that take time to set up and can only process one sample at a time
  • Easy to use—no cumbersome column preparation or equilibration
  • Flexible formats—available in spin columns and 96-well spin plates for a range of sample volumes (2 μL to 10 mL)

Zeba Desalting Columns

Convenient, ready-to-use polypropylene columns are the ideal solution for quick and straightforward desalting and buffer exchange of protein samples in a centrifuge format. The pre-dispensed spin columns are offered in various resin fills (75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL, 20mL). These options make it simple to handle sample volumes between 2 μL and 10 mL.

Zeba Spin Desalting Plates

These plates provide trouble-free high-throughput desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. They require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes. Separating macromolecules, such as proteins, from small contaminants is often done through routine methods, including gel filtration chromatography and buffer exchange. These methods are commonly used during protein purification workflows to remove small contaminants like salts, excess labels, and cross-linking reagents from samples before analysis.

Specifications
DisposableSingle-use
For Use With (Application)Small molecule removal and buffer exchange
For Use With (Equipment)Standard Table-top Centrifuge
Formulation20 mL Zeba Desalting Resin Bed
Purification TargetBuffer Exchange, Protein
Quantity10 Columns
Volume (Metric)20 mL
Column TypeSize-exclusion, Proprietary Resin
FormatSpin Column
MWCO40 kDa
Product LineZeba™
Sample Volume (Metric)4 to 10 mL
Unit Size10 columns
Contents & Storage
Store at 4°C

Frequently asked questions (FAQs)

Can Zeba Desalting columns, plates, and cartridges be autoclaved?

Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?

Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

With Zeba Spin Desalting columns, plates, and cartridges, how can I completely get rid of glycerol and detergent from my sample?

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although other methods are a better choice for removing these components, Zeba columns can be used by modifying the desalting protocol. Modifications include diluting the sample, processing a smaller volume of the sample, and adding the sample slowly to allow it to enter the gel and equilibrate into the pores before processing.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

After desalting with your Zeba Spin Desalting columns, I still have leftover salt in my sample. What should I do to get rid of all the salt?

You can either reduce the volume of sample processed in each column (the product manual indicates the volume range recommended for each column size) or run the flow-through from one column over a fresh column.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can Zeba Spin Desalting columns, plates, and cartridges be used for glycerol and detergent removal?

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although other methods are a better choice for removing these components, Zeba columns can be used by modifying the desalting protocol. Modifications include diluting the sample, processing a smaller volume of the sample, and adding the sample slowly to allow it to enter the gel and equilibrate into the pores before processing.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.