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Citations & References (53)
Invitrogen™
4-Acetamido-4'-Maleimidylstilbene-2,2'-Disulfonic Acid, Disodium Salt
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonatedRead more
| Catalog Number | Quantity |
|---|---|
| A485 | 25 mg |
Catalog number A485
Price (CNY)
5,015.00
Each
Quantity:
25 mg
Price (CNY)
5,015.00
Each
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityThiol
Label or DyeStilbene
Product Type4-Acetamido-4'-Maleimidylstilbene-2,2'-Disulfonic Acid
Quantity25 mg
Reactive MoietyMaleimide
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).
Citations & References (53)
Citations & References
Abstract
AppA is a blue light photoreceptor that antirepresses photosynthesis gene expression in Rhodobacter sphaeroides.
Journal:Cell
PubMed ID:12230978
Photosynthetic bacteria regulate photosystem synthesis in response to alterations in oxygen tension and light intensity. In this study we show that the PpsR repressor from Rhodobacter sphaeroides binds to DNA in a redox-dependent manner through the formation/breakage of an intramolecular disulfide bond. We also demonstrate that PpsR is antagonized by
Protein-protein interactions between UDP-glucuronosyltransferase isozymes in rat hepatic microsomes.
Journal:Biochemistry
PubMed ID:9188715
'The interactions between UDP-glucuronosyltransferase (UGT) isozymes, UGT1s and UGT2B1, in rat hepatic microsomes were investigated using an immunopurification technique with anti-peptide antibodies and a chemical cross-linking strategy. A 50 kDa protein coimmunopurified with UGT1s was identified as UGT2B1 by amino-terminal sequencing and immunodetection with anti-peptide antibody against UGT2B1. Evidence for
Extracellular DsbA-insensitive folding of Escherichia coli heat-stable enterotoxin STa in vitro.
Journal:J Biol Chem
PubMed ID:10744753
'To study the folding of human Escherichia coli heat-stable enterotoxin STh, we used the major protein subunit of CS31A fimbriae (ClpG) as a marker of STh secretion and a provider of a signal peptide. We established that STh genetically fused to the N or C terminus of ClpG was able
Investigation of the extracellular accessibility of the connecting loop between membrane domains I and II of the bradykinin B2 receptor.
Journal:J Biol Chem
PubMed ID:10329674
'In analogy to the structure of rhodopsin, the seven hydrophobic segments of G-protein-coupled receptors are supposed to form seven membrane-spanning alpha-helices. To analyze the topology of the bradykinin B2 receptor, we raised site-directed antibodies to peptides corresponding to the loop regions and the amino and carboxyl terminus of this receptor.
Cysteine-scanning mutagenesis reveals a highly amphipathic, pore-lining membrane-spanning helix in the glutamate transporter GltT.
Journal:J Biol Chem
PubMed ID:11148213
'The carboxyl-terminal membrane-spanning segment 8 of the glutamate transporter GltT of Bacillus stearothermophilus was studied by cysteine-scanning mutagenesis. 21 single cysteine mutants were constructed in a stretch ranging from Gly-374 to Gln-404. Two mutants were not expressed, four were inactive, and two showed severely reduced glutamate transport activity. Cysteine mutations