Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.
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Catalog Number
Quantity
A49784
1 mL
A51218
6 x 1 mL
2 Options
Catalog number A49784
Price (CNY)
-
Quantity:
1 mL
Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.
Adapted to the chemically defined Viral Production Medium and maintained in suspension culture in that medium
Highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
Supplied frozen in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
Should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
Clonal, 293F-derived, high-producer cell line
Absence of SV40 large T antigen
Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
Robust scalability and passage stability
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell StateFrozen
Concentration1 x 107 cells/mL
For Use With (Application)AAV Production
Format1 Vial
Media RecommendationGibco Viral Production Medium
No. of Cells1 x 107 cells
Product LineAAV-MAX
Product TypeViral Production Cells
Quantity1 mL
Shipping ConditionDry ice for domestic shipment; liquid nitrogen for international shipment
SourceHuman
Cell LineViral Production Cells 2.0
Cell TypeKidney (Embryonic)
Culture TypeSuspension Cell Culture
FormFrozen
SpeciesHuman
Unit SizeEach
Contents & Storage
1 cryovial, store in vapor phase liquid nitrogen
Frequently asked questions (FAQs)
Can Viral Production Cell 2.0 (Cat. No. A49784, A51218) only grow in suspension, or can they be adherent using specific medium conditions?
Viral Production Cells 2.0 were designed specifically for suspension cultures. We did not validate their usage with adherent culture and therefore do not recommend doing so.
Can I use Viral Production Cells (Cat. No. A35347, A35827) with the AAV MAX Helper-Free AAV Production System?
The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.
What is the highest density that Viral Production Cells 2.0 can be cultured at?
The Viral Production Cells 2.0 achieve maximum cell densities of >1.2 x 10E7 cells/mL in shake flask cultures.
What is the passage stability of the Viral Production Cells 2.0?
We recommend using the Viral Production Cells 2.0 between passages 3 to 25 for adeno-associated virus (AAV) production.
How long do the Viral Production Cells 2.0 require to recover post-thaw before transfection?
We recommend allowing freshly thawed Viral Production Cells 2.0 to recover in culture for at least three passages prior to transfection.