Expi293™ Flex Expression Medium, 1 L

Gibco Expi293 Flex Expression Medium is a chemically defined, serum-free, protein-free medium for suspension-adapted human embryonic kidney (HEK)293 cell cultivation and transfection. Serving as a key component of the Expi293 Flex Expression System, it enables high-density culture of Expi293 Flex cells and scalable transient protein production.

• Enables suspension HEK 293 cell cultures to reach cell densities exceeding 15 x 10⁶ cells/mL.
• Serum-free suspension cell culture medium compatible with transfection
• Supports sustained, high expression in high-density transient transfection cultures, with recombinant protein yields reaching 5 g/L maximum
• Compatible with HEK293 cell cultivation and transfection across culture volumes ranging from 1 mL to 10 L
• Does not contain phenol red

Expi293 Flex Expression Medium incorporates GlutaMAX‐I reagent and is supplied as a ready‐to‐use formulation requiring no additional supplements. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Flex Expression Medium is free of human and animal‐derived components and is not intended for adherent cell culture applications.