AAV-MAX Lysis Buffer

Gibco AAV-MAX Lysis Buffer is a ready-to-use, chemically defined cell lysis reagent optimized for adeno-associated virus (AAV) vector production workflows. It uses a gentle, detergent-based formulation to eliminate the need for mechanical cell disruption, providing a milder and easier alternative for isolating AAV particles from HEK293 cell cultures. Supplied as a 10X concentrate, it can be directly added to HEK293 production cultures to induce lysis, streamlining AAV particle extraction and recovery from upstream workflows. AAV-MAX Lysis Buffer integrates seamlessly with the AAV-MAX Production System while remaining compatible with other AAV vector production processes, enabling flexible use across different AAV workflows.

• Ready-to-use 10X solution—convenient concentrated format can be added directly to cell culture, with no dilution or premixing required
• Detergent-based cell lysis—gentle chemical lysis avoids mechanical disruption (no sonication or freeze-thaw needed), simplifying AAV particle recovery from cells
• High-yield AAV extraction—optimized for efficient HEK293 cell lysis for AAV production, maximizing AAV particle recovery and supporting high titers of viral vectors
• Broad workflow compatibility—functions with the AAV-MAX Production System, from research scale to bioproduction, for versatile application
• Chemically defined formulation—Polysorbate 20-based, animal-origin free buffer (Triton™ X-100–free) for consistent performance and regulatory compliance