Efficient-Pro™ AGT™ Feed 1
Efficient-Pro™ AGT™ Feed 1
Gibco™

Efficient-Pro™ AGT™ Feed 1

Efficient-Pro AGT Feed 1 is a next-generation Chinese hamster ovary (CHO) feed used in the production of monoclonal antibodies (mAbs),Read more
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Catalog NumberQuantity
A52091011 L
A520910210 L
A5209103100 L
Catalog number A5209101
Price (CNY)
5,323.00
Each
Add to cart
Quantity:
1 L
Price (CNY)
5,323.00
Each
Add to cart
Efficient-Pro AGT Feed 1 is a next-generation Chinese hamster ovary (CHO) feed used in the production of monoclonal antibodies (mAbs), offering significant performance and ease-of-use improvements over earlier Gibco and competitor products. Designed to work optimally with Efficient-Pro AGT Medium, Efficient-Pro AGT Feed 1 has been formulated specifically for use in CHO-K1 cell lines. The Efficient-Pro AGT system increases titer and specific productivity, improves protein quality and metabolite profiles, and is easier to use with less solubility issues than other CHO media and feed offerings.

The Efficient Pro AGT system will help you:
• Benefit from the ease-of-use associated with Gibco’s trademarked, patented Advanced Granulation technology (AGT) for auto-pH and simplified dissolution
• Sustain cell viability for longer bioreactor runs and maximized titer
• Improve downstream outputs such as protein glycosylation, charge-variants, and aggregation
• Speed up process development and streamline transfer to manufacturing scale
• Increase flexibility with a range of format and packaging configurations
• Decrease cell shock with simplified adaptation protocols

Efficient-Pro AGT Feed 1 has been formulated as a chemically defined, animal-origin-free cell culture feed used in the bioproduction of mAbs and other therapeutics in traditional batch and fed-batch bioprocessing workflows, particularly in combination with CHO cell line variants.

Efficient-Pro AGT Feed 1 is a balanced formulation designed to meet the metabolic needs of mAb production and does not contain growth factors or insulin to ensure batch-to-batch consistency and production efficiency. Demonstrated to improve productivity in a CHO-K1 cell line when paired with Efficient-Pro AGT Medium compared to an industry-leader’s media and feed system, the Efficient-Pro AGT system helps accelerate your time-to-market and achieve success.

For research use only. Not for use in diagnostic procedures.
Specifications
Cell LineCHO-DG44, CHO-S, CHO-K1, GS-CHO
Culture TypeMammalian Cell Culture
Green FeaturesSustainable packaging
Quantity1 L
Shipping ConditionRoom temperature
ClassificationAnimal Origin-free, Chemically-defined, Protein-free, Serum-free
FormAGT
Unit SizeEach
Contents & Storage
Store in the dark at 2–8°C.

Frequently asked questions (FAQs)

Can I use Efficient-Pro AGT Feed 1 with other cell lines besides CHO-S and DG-44 cell line variants?

Efficient-Pro AGT Feed 1 has been designed specifically for CHO-S and DG-44 cells; however, it has been demonstrated to improve performance in other CHO variants and even other parental cell lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I currently supplement my CHO cells with insulin and other growth factors. Should I continue the use of these other additives when using Efficient-Pro AGT Feed 1?

We recommend continuing current supplementation protocols including insulin and other growth factors when using Efficient-Pro AGT Feed 1.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf-life of Efficient-Pro AGT Feed 1?

The shelf-life of Efficient-Pro AGT Feed 1 is 12 months from the date of manufacture. The expiration date is provided on the product label.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How often and at what concentration should I feed my CHO cells with Efficient-Pro AGT Feed 1?

The comprehensive feed protocol for Efficient-Pro AGT Feed 1 is included in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025907_EfficientProAGTFeed1_UG.pdf). We recommend first designing a small-scale experiment using an Ambr15 Microbioreactor or other small vessel with concentrations between 1.5% to 3.0% of Efficient-Pro AGT Feed 1 in 0.5% increments beginning on day 3, with daily feeds through harvest. Once the specific feeding strategy is tailored to your CHO cell system for optimized results, cultures can be scaled up to larger vessels and bioreactors.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When should I consider using Efficient-Pro AGT Feed 1?

Efficient-Pro AGT Feed 1 is a chemically-defined, animal-origin-free supplement that has been formulated to work specifically with CHO-S and DG-44 cells for production of proteins. Efficient-Pro AGT Feed 1 has been designed to work optimally when paired with Efficient-Pro AGT Medium (https://www.thermofisher.com/order/catalog/product/A5322301). Efficient-Pro AGT Feed 1 has been demonstrated to improve yield and other end-points when used as described in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025907_EfficientProAGTFeed1_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.