QuantStudio™ Absolute Q™ Digital PCR System, desktop

The Applied Biosystems QuantStudio Absolute Q Digital PCR System combines the power of digital PCR (dPCR) absolute quantification with theRead more

Catalog Number	Quantity
A52864	1 system

Catalog number A52864

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1 system

The Applied Biosystems QuantStudio Absolute Q Digital PCR System combines the power of digital PCR (dPCR) absolute quantification with the simplicity of a qPCR workflow. With a single hands-on step that takes five minutes to complete, dPCR is now easier than ever. The QuantStudio Absolute Q system provides a high quality, consistent, and easy-to-use solution for researchers who want to join the quantification revolution.

Features of the QuantStudio Absolute Q system include:
• Easy and convenient qPCR-like workflow with only five minutes of hands-on time
• Reduced errors and manual inputs with all functions integrated into a single instrument
• Increased productivity with results in as little as 90 minutes
• Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
• QuantStudio Absolute Q Digital PCR software enables intuitive setup, monitoring, and analysis
- Security, auditing, and e-signature features for 21CFR part 11-compliance support
• Compatible with Absolute Q Liquid Biopsy Digital PCR assays for oncology applications, as well as our portfolio of TaqMan assays for gene expression, genetic variation, gene regulation, and other quantification experiments

Easy and convenient workflow
The QuantStudio Absolute Q system was designed for a simple and powerful dPCR experience. All the necessary steps for dPCR—compartmentalization, thermal cycling, and data acquisition—are integrated into a single instrument. The simple qPCR-like workflow can be completed with under 5 minutes of hands-on time.

Accomplish more, faster
The QuantStudio Absolute Q system can complete a run in less than 90 minutes.

Results you can trust
Consumable plates with patented microfluidic array technology allow for over 95% of the sample to be analyzed across over 20,000 consistently generated microchambers for optimal quantification of even rare targets.

Application versatility
With the ability to multiplex using up to four optical channels, the QuantStudio Absolute Q system enables more targets to be measured per sample, saving time and reagents. The Absolute Q dPCR platform is an ideal solution for any application requiring high sensitivity, precision, and accuracy.

More information (including application notes) >
QuantStudio Absolute Q assays >

Powerful and intuitive software
Absolute Q dPCR software is designed to complement our intuitive workflow to save time and effort. Parameters such as plate format, optical channels, and thermal conditions can be easily modified to start generating data quickly.

The clean and powerful data analysis interface provides a range of options for processing data. Whether visualizing multiplexed data or calculating concentrations across replicates, results can be exported for downstream analysis or compiled into an easy-to-read report in just a few clicks.

Included security, auditing and e-signature software features support compliance with 21CFR part 11 for regulated environments.

Peace of mind with Smart service
Minimize downtime with premium service offerings for the QuantStudio Absolute Q Digital PCR System. Smart Remote Support is an immersive collaboration tool that allows customers to quickly and efficiently show issues to Technical Support for resolution, helping reduce instrument downtime compared to on-site repair and helping minimize the need for on-site visits.

Learn more about available financing options and programs ›

For Research Use Only. Not for use in diagnostic procedures

Specifications

For Use With (Application)Digital PCR

For Use With (Equipment)QuantStudio Absolute Q MAP16 Plate

Product LineQuantStudio

Quantity1 system

Shipping ConditionRoom Temperature

Block FormatNon-interchangeable

Unit SizeEach

Contents & Storage

QuantStudio Absolute Q Digital PCR System, Desktop

Frequently asked questions (FAQs)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is the limit of detection for the QuantStudio Absolute Q Digital PCR System (Cat. No. A52864)?

The QuantStudio Absolute Q Digital PCR Instrument (Cat. No. A52864) uses QuantStudio Q MAP16 Digital PCR Plates that contain 16 digital PCR microreaction chamber arrays, each containing 20,480 fixed volume microreaction chambers of 432 ρL (0.000432 µL). If there is 1 copy of gene target in the tested sample, and the sample is using 1 array of the MAP16 plate, then the theoretical lower limit of detection (lowLOD) for the Absolute Q can be approximated by the following calculation:

lowLOD for Absolute Q = (1 copy)/ (0.000432 µL X 20,480 chambers) = 0.113 copies per µL

If a sample is run in triplicate, and there are 3 positive chambers, the lowLOD in practice for Absolute Q can be calculated using the following calculation:

lowLOD for Absolute Q = (3 copies)/ (0.000432 µL X 20,480 chambers) = 0.339 copies per µL

The QuantStudio Absolute Q has the sensitivity of detecting the presence of a single copy of target sequence within the 9 µL reaction volume, which would result in a detected concentration of ~0.1 copies per µL. This detection limit can be reduced even further by using the software’s digital pooling feature to increase the total analyzed volume. The ability of the instrument to detect at this level will be impacted by various components including the assay efficiency and reagents used in the reaction. The LOD is also dependent on a given assay’s specificity as well as the potential background fluorescence signal from the sample matrix, which may affect the LOD achieved in practice. The Absolute Q software’s unique false positive detection capability can enhance an assay’s performance, especially when low target concentrations are investigated.

It is important to note that the LOD should be determined empirically by the user for their specific experimental conditions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What optical dyes does QuantStudio Absolute Q Digital PCR System (Cat. No. A52864) support?

QuantStudio Absolute Q Digital PCR System (Cat. No. A52864) supports the following dyes: FAM™ dye, VIC™ dye, HEX™ dye, ABY™ dye, ROX™ dye, CY™5 dye, and JUN™ dye.

Please refer to page 13 of the QuantStudio Absolute Q Digital PCR System User Guide for Installation, Use and Maintenance for more information on supported optical dyes and channel selection.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I finished running my experiment on the QuantStudio Absolute Q Digital PCR instrument but just realized that I had selected the wrong dyes during setup. Can I change the dyes that were used after the run?

If dye channels are turned off before the run, then data will not be collected for them as the run progresses. This means that changing the dyes after the run will not be possible.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I ran my experiment using the QuantStudio Absolute Q Digital PCR System but did not actually use dyes in all the channels. Is there a way to only see those dye channels that were used?

Unused dye channels can be turned off after the run by going to the SETUP tab and clicking EDIT SETUP and then EDIT GROUPS. Here, the Analysis can be changed to “Not Used.” After clicking SAVE twice, the data will be reanalyzed and the unused dye channels will not be present in the analysis.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

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