QuantStudio™ Absolute Q™ MAP16 Plate Kit and Master Mix

Learn more about our Absolute Q Universal DNA Digital PCR Master Mix. Seamless switch. Broader compatibility with applications. Backed by 20M+ Applied Biosystems predesigned TaqMan Assays, performance-guaranteed*

SKU A53301 is being discontinued in December but the components of the SKU A53301, the QuantStudio Absolute Q MAP16 Plate Kit (Cat. Number A52865) and Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) (Cat. Number A52490) will be available as standalone products for purchase.

Applied Biosystems™

QuantStudio™ Absolute Q™ MAP16 Plate Kit and Master Mix

The Applied Biosystems QuantStudio Absolute Q MAP16 Plate Kit is a key component of the QuantStudio Absolute Q Digital PCRRead more

Catalog Number	Quantity
A53301	12 plates

Catalog number A53301

Price (CNY)

Quantity:

12 plates

The Applied Biosystems QuantStudio Absolute Q MAP16 Plate Kit is a key component of the QuantStudio Absolute Q Digital PCR System. The system is powered by the patented microfluidic array plate (MAP) technology that enables consistent and automated separation of the sample into 20,000 microchambers with industry leading <1% coefficient of variation (CV). The QuantStudio Absolute Q MAP16 consumable uses microinjection-molded plates to overcome common challenges of inconsistency and high dead volume. This novel approach provides many benefits including: precise microchamber volume, consistent sample distribution, and greater than 98% of loaded volume analyzed.

This package product includes QuantStudio Absolute Q DNA Master Mix, which is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps. The 5X formulation enables analysis of higher sample volumes and delivers accurate quantification of DNA targets without using a standard curve. Learn more about this master mix

The unparalleled precision and accuracy of the Absolute Q system provides confidence in measurements across replicates and experiments.

For Research Use Only. Not for use in diagnostic procedures

Specifications

For Use With (Application)Digital PCR

Includes12 MAP16 Plates, Gaskets, Isolation Buffer, and Absolute Q™ DNA Digital PCR Master Mix

Quantity12 plates

Unit SizeEach

Frequently asked questions (FAQs)

I finished running my experiment on the QuantStudio Absolute Q Digital PCR instrument but just realized that I had selected the wrong dyes during setup. Can I change the dyes that were used after the run?

If dye channels are turned off before the run, then data will not be collected for them as the run progresses. This means that changing the dyes after the run will not be possible.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I ran my experiment using the QuantStudio Absolute Q Digital PCR System but did not actually use dyes in all the channels. Is there a way to only see those dye channels that were used?

Unused dye channels can be turned off after the run by going to the SETUP tab and clicking EDIT SETUP and then EDIT GROUPS. Here, the Analysis can be changed to “Not Used.” After clicking SAVE twice, the data will be reanalyzed and the unused dye channels will not be present in the analysis.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I'm using the QuantStudio Absolute Q Digital PCR System and the software is saying that the concentration is NaN. What does that mean?

NaN stands for “not a number”. The software will display NaN instead of an actual number when it has detected some issue during the analysis of the array images.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

With the QuantStudio Absolute Q Digital PCR instrument, the run data didn't automatically transfer after the run completed. How can I get the data?

We would recommend restarting the software. If the run still doesn't transfer, we would recommend closing the software, rebooting the instrument, and then starting the software. If further assistance is needed, please contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

With the QuantStudio Absolute Q Digital PCR System, how much sample can be loaded into each well?

9 µL of sample is loaded into each well along with 15 µL of isolation buffer. Loading more or less of either the sample or isolation buffer will affect filling of the microchambers and the run results.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all QuantStudio™ Absolute Q™ MAP16 Plate Kit and Master Mix FAQs