TaqMan™ QK Platinum Multiplex Master Mix

TaqMan QK Platinum Multiple Master Mix is specially designed for fast, multiplex, high-sensitivity real-time PCR detection with high-quality results for even complex samples. This master mix contains UDG enzyme to help prevent crossover contamination, and with high stability and batch-to-batch consistency, it is ideal for biomedical applications and molecular diagnostics.

Features of TaqMan QK Platinum Multiple Master Mix include:

• High sensitivity—amplifies DNA targets down to five copies
• Multiplexed targets—can detect up to five targets including exogenous and endogenous controls
• Quick response—40 minutes in quick mode using QuantStudio 5 system
• High stability—prepared reactions can be stored for 72 hours at room temperature
• Batch-to-batch consistency—production process follows ISO 9001 quality management system to ensure consistency between production batches
• Wide dynamic range—accurately measure up to 6 logs of dynamic range

High sensitivity

TaqMan QK Platinum Multiple Master Mix has been optimized for high-sensitivity detection of low-abundance targets. As few as five copies of DNA template have been detected in a 20-μL reaction (see figure below).

Multiple targets

TaqMan QK Platinum Multiple Master Mix can accommodate up to five channels of simultaneous detection. A figure below shows linear regression results of 5-plex reactions of human cDNA for ACADM, APOE, RNaseP, GAPDH, and an exogenous internal positive control (IPC), with an R2 value greater than 0.98.

Fast response

TaqMan QK Platinum Multiple Master Mix enables detection in ∼40 minutes in fast mode on a QuantStudio 5 Real-Time PCR System. It is suitable for use on rapid and standard instruments, enabling researchers who currently have standard instruments to reap the performance benefits and time savings provided by this master mix.

High stability

TaqMan QK Platinum Multiple Master Mix was engineered to maintain high levels of performance in prepared reactions for up to 72 hours at room temperature. A figure below shows the stability of reactions performed at the time of preparation (time 0) and after 72 hours of incubation at room temperature.

High batch-to-batch consistency

The production process of TaqMan QK Platinum Multiple Master Mix follows the ISO 9001 quality management system, which helps ensure the traceability of the production process and establishment of quality control points from raw material procurement to product release, helping produce high batch-to-batch consistency of the master mix (see figure below).

Wide dynamic range

TaqMan QK Platinum Multiple Master Mix can yield up to 6 logs of dynamic range (see figure below) and shows equivalent or better dynamic range in the detection of various targets compared to other master mixes (see figure below).