Why do the iBright Imaging Systems use green LED illumination for DNA imaging?
The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer several additional benefits:
- No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.
- No mercury waste: UV transilluminator bulbs may contain mercury, a hazardous substance, and therefore require special care for handling and disposal.
- Longer lifetime: LED bulbs have a substantially longer real-time life than fluorescent UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.
For additional information on iBright functionality, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
What sample types can I image with the iBright CL750 Imaging System?
The iBright CL750 Imaging System supports imaging of chemiluminescent western blots, colorimetric and fluorescent stained gels, and nucleic acid gels. It can also be used to image GFP- and crystal violet-stained colony plates.
For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
What sample types can I image with the iBright CL1500 Imaging System?
The iBright CL1500 Imaging System supports a wide range of applications including the following:
Protein gel: colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)
Nucleic acid gel: ethidium bromide and Invitrogen SYBR stains; imaging of E-Gels and hand pour gels is supported
Chemiluminescent western blot: chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Additional applications: colony counting for GFP, crystal violet, and blue/white colonies; TLC plates, and leaf sections
For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
What sample types can I image with the iBright FL1500 Imaging System?
The iBright FL1500 Imaging System provides maximum application support including up to 4-channel multiplex western blot imaging. See the list below for more details:
Protein gel: colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)
Nucleic acid gel: ethidium bromide and Invitrogen SYBR stains; imaging of E-Gels and hand-poured gels is supported
Chemiluminescent western blot: chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Fluorescent western blot: fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates)
Additional applications: colony counting for GFP, crystal violet, and blue/white colonies; TLC plates, and leaf sections
For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
