Custom TaqMan™ Gene Expression Assays

Applied Biosystems Custom TaqMan Gene Expression assays are the custom version of our predesigned TaqMan assays for quantitative real-time PCR. The Custom TaqMan Gene Expression assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM, VIC, ABY, JUN, and Cyanine 5) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

Features include:

• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
• Sequences provided—with every Custom TaqMan Gene Expression Assay you design and order
• Multiplexing ready—TaqMan assays are now available with 5 different dye labels to support simultaneous detection of up to 5 targets in a single qPCR reaction. FAM, ABY, JUN, and Cyanine 5 assays are available with balanced primer/probe concentrations (Primer-Limited, 350 nM) to support consistent multiplex performance. VIC Primer-Limited assays (150 nM) are available with SKU 4448487 (S), 4448488 (M), 4448492 (L).

Approximate ship time

• Made to order: 4–6 days in North America, 4–8 days in Europe (FAM, VIC); 4-10 days in North America, 6-14 days in Europe (ABY, JUN, Cyanine 5)

Pre-formulated assay

• 2 unlabeled PCR primers (Standard: 900 nM each final 1X concentration; Primer-Limited: 350 nM each final 1X concentration )
• 1 dye-labeled (FAM, VIC, ABY, JUN or Cyanine 5) TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Recommended master mix: TaqMan Fast Advanced Master Mix (Cat. No. 4444556) or TaqPath DuraPlex 1-Step RT-qPCR Master Mix (Cat. No. A58666)

See also: Custom Plus TaqMan RNA Assays, designed using the full bioinformatic power of the TaqMan Assay design pipeline.